公开(公告)号：EP2986957A4

公开(公告)日：2017-03-15

申请号：EP15785338

申请日：2015-04-29

Applicant: BIO-RAD LABORATORIES INC

Inventor： THRUSH EVAN ,  SWIHART STEVE ,  BHATT NEERAJ

IPC: G01J3/30 ,  F21V9/00 ,  G01N21/64

CPC classification number: G01N21/6456 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2021/6471 ,  G01N2021/6482 ,  G01N2201/062 ,  G01N2201/0627 ,  G01N2201/0638 ,  G01N2201/12

Abstract: An imaging assembly and processing system that includes a sample platform having a target region which can hold a sample, where the sample can be marked with fluorescent or phosphorescent markers. The imaging assembly can have an excitation light module proximate to the sample platform that emits light to excite the markers, and a lens module positioned to receive emission light from excited markers in target region. At least one series filter assembly or interference filter can be arranged in front of, behind, or both in front of and behind the lens module. The assembly includes a light sensor and a processor and imaging module configured to process data captured by the light sensor. Images of the sample are generated based on the emission light from the sample that transmit through and are filtered by the lens assembly and series filter assembly or interference filter.

公开(公告)号：EP2989443A4

公开(公告)日：2016-12-21

申请号：EP14869916

申请日：2014-12-09

Applicant: BIO-RAD LABORATORIES INC

Inventor： SWIHART STEVE ,  THRUSH EVAN ,  RAN BOAZ

IPC: G01N21/27 ,  G01N21/64 ,  G01N21/76 ,  H04N5/217 ,  H04N5/235 ,  H04N5/355 ,  H04N5/361

CPC classification number: H04N5/2355 ,  G01N21/272 ,  G01N21/6408 ,  G01N21/6456 ,  G01N21/76 ,  H04N5/2173 ,  H04N5/2353 ,  H04N5/35545 ,  H04N5/361 ,  H04N5/365

Abstract: Methods and digital imaging devices disclosed herein are adapted to capture images of a specimen in a chemical reaction using a series of short exposures of light emissions from the specimen over a period of time. The series of short exposures is captured using an array of pixels of an image sensor in the digital imaging device that are configured for performing continuous non-destructive read operations to read out a set of non-destructive read images of the specimen from the pixel array. In one embodiment, images are captured by delaying the read out until at or near the end of the chemical reaction to reduce read noise in the images. The signals read out from the image sensor can be continuously monitored and the capturing of images can be discontinued either automatically or based on a command from a user. The captured images can then be displayed in a graphical display.

Abstract translation: 本文公开的方法和数字成像装置适于在一段时间内使用来自样品的一系列短的曝光来捕获化学反应中的样本的图像。 使用数字成像装置中的图像传感器的像素阵列来捕获一系列短曝光，其被配置为执行连续的非破坏性读取操作，以从像素阵列读出样本的一组非破坏性读取图像 。 在一个实施例中，通过延迟读出直到化学反应结束或接近化学反应结束来捕获图像，以减少图像中的读取噪声。 可以连续地监视从图像传感器读出的信号，并且可以自动地或基于来自用户的命令中断图像的捕获。 然后可以在图形显示中显示所捕获的图像。