公开(公告)号：CA2721582C

公开(公告)日：2017-07-11

申请号：CA2721582

申请日：2009-04-17

Applicant: BIO-RAD LABORATORIES INC

Inventor： WATKINS MICHAEL I ,  MARR GREGORY A ,  YANG XIAOYUN ,  BRUEHL RICHARD ,  SHAN DAMING ,  COLEMAN PATRICK F

IPC: C12N15/29 ,  A61K38/00 ,  C07K14/415 ,  C07K19/00 ,  C12N15/63 ,  G01N33/53 ,  G01N33/564 ,  G01N33/68

Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.