公开(公告)号：JP2012228257A

公开(公告)日：2012-11-22

申请号：JP2012151968

申请日：2012-07-06

Applicant: Basf Se ,  ビーエーエスエフ ソシエタス・ヨーロピアBasf Se

Inventor： BREUER MICHAEL ,  HAUER BERNHARD ,  SCHWANEBERG ULRICH ,  WONG TUCK SENG

IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N9/16 ,  C12P41/00

CPC classification number: C12N9/16 ,  C12P7/62 ,  C12P41/003

Abstract: PROBLEM TO BE SOLVED: To provide novel proteins exhibiting esterase activity and mutants thereof, nucleic acid sequences coding therefor, expression cassettes, vectors and recombinant microorganisms.SOLUTION: There are disclosed a method for producing the proteins obtained by starting from Pseudomonas glumae (Burkholderia plantarii) LU 2023 and the use of the proteins for the enzymatic, in particular, enantioselective ester hydrolysis or interesterification of organic esters.

Abstract translation: 要解决的问题：提供表现出酯酶活性的新蛋白质及其突变体，编码它们的核酸序列，表达盒，载体和重组微生物。 解决方案：公开了一种生产通过从假单胞菌（Burkholderia plantarii）LU 2023开始获得的蛋白质的方法，以及使用蛋白质进行酶促，特别是有机酯的对映选择性酯水解或酯交换。 版权所有（C）2013，JPO＆INPIT