公开(公告)号：EP2799844A1

公开(公告)日：2014-11-05

申请号：EP13871421.7

申请日：2013-08-08

Applicant: Beijing Institute Of Technology

Inventor： ZHAO, Weiqian ,  CUI, Han ,  QIU, Lirong ,  WANG, Yun

IPC: G01N21/65 ,  G01N21/47

CPC classification number: G01N21/47 ,  G01N21/65 ,  G01N21/658 ,  G01N2201/063

Abstract: The present invention belongs to a technical field of optical microscopic imaging and spectral measurement, and discloses a laser differential confocal mapping-spectrum microscopic imaging method and device. The core concept of the present invention is to combine the differential confocal detection and the spectrum detection techniques and use a dichroic beam splitting system (13) to separate the Rayleigh light for geometric position detection from the Raman scattering light for spectrum detection, by mean of the property that the zero-cross point of the differential confocal curve (43) accurately corresponds to the focus of the objective, the spectral information at focus of the excitation spot being accurately captured by a zero trigger to accomplish the spectrum detection with high spatial resolution. Therefore, the present invention provides a method and device that may be able to accomplish the spectrum detection with high spatial resolution to a micro-area of a sample. The advantages of the present invention include accurate positioning, high spatial resolution, high spectrum detection sensitivity and controllable size of measuring converging spot, and etc. Therefore, the present invention has broad application prospects in biomedical, forensic and other fields.

Abstract translation: 本发明属于光学显微成像和光谱测量的技术领域，并且公开了一种激光微分共焦映射光谱显微成像方法和装置。 本发明的核心概念是组合差分共聚焦检测和光谱检测技术，并使用二向色分束系统（13）将用于几何位置检测的瑞利光与用于光谱检测的拉曼散射光分离，通过 差分共聚焦点曲线（43）的零交叉点与物镜的焦点精确对应的特性是，通过零触发准确地捕获激发光点焦点处的光谱信息，以实现高空间分辨率的光谱检测 。 因此，本发明提供了一种能够以高空间分辨率对样品的微区进行光谱检测的方法和装置。 本发明的优点包括精确定位，高空间分辨率，高光谱检测灵敏度和测量聚光点的可控尺寸等。因此，本发明在生物医学，法医学等领域具有广阔的应用前景。