公开(公告)号：US20040229355A1

公开(公告)日：2004-11-18

申请号：US10437627

申请日：2003-05-14

Applicant: Board of Regents University of Texas System

Inventor： Ming Chen ,  Iwona Stroynowski

IPC: C12N005/08 ,  C12N005/02

CPC classification number: C12N5/067 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2501/11 ,  C12N2501/39

Abstract: A culture medium, which is capable of sustaining long-term cultures of hepatocytes and liver cells. In this medium, mammalian primary hepatocytes retain highly replicative capacity and hepatic gene expression activity. The liver cells from genetically defined sources may be reproducibly immortalized without the delivery of foreign genes, such as viral oncogenes. The immortalized hepatocytes are non-tumorigenic, making them suitable for clinical and therapeutic purposes.

Abstract translation: 一种能够维持肝细胞和肝细胞长期培养的培养基。 在该培养基中，哺乳动物原代肝细胞具有高度复制能力和肝基因表达活性。 来自遗传定义的来源的肝细胞可以可再现地永生化，而不会输送外源基因，如病毒癌基因。 永生化肝细胞是非致瘤性的，使其适合临床和治疗目的。