公开(公告)号：WO2005017116A3

公开(公告)日：2005-05-06

申请号：PCT/US2004026248

申请日：2004-08-11

Applicant: CALIFORNIA INST OF TECHN ,  ARNOLD FRANCES H ,  CIRINO PATRICK C

Inventor： ARNOLD FRANCES H ,  CIRINO PATRICK C

IPC: C07H21/04 ,  C12N20060101 ,  C12N5/06 ,  C12N9/02 ,  C12P21/02

CPC classification number: C12N9/0077

Abstract: The invention relates to novel variants of cytochrome P450 oxygenases. These variants have at least one mutation improving their ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. The variants also have at least one mutation improving thermostability as compared to the parent enzyme or corresponding wild-type enzyme. Preferred variants include cytochrome P450 BM-3 heme domain variants having L52I, I58V, F87A, H100R, S106R, F107L, A135S, M145A/V, A184V, N239H, S274T, L324I, V340M, I366V, K434E, E442K, and/or V446I amino acid substitutions.

Abstract translation: 本发明涉及细胞色素P450加氧酶的新变体。 与相应的野生型酶相比，这些变体至少有一个突变提高了它们使用过氧化物作为氧供体的能力。 与亲本酶或相应的野生型酶相比，所述变体还具有至少一个改善热稳定性的突变。 优选的变体包括具有L52I，I58V，F87A，H100R，S106R，F107L，A135S，M145A / V，A184V，N239H，S274T，L324I，V340M，I366V，K434E，E442K和/或V446I的细胞色素P450 BM- 氨基酸取代。

公开(公告)号：EP1470219A4

公开(公告)日：2005-10-05

申请号：EP02762117

申请日：2002-04-16

Applicant: CALIFORNIA INST OF TECHN

Inventor： CIRINO PATRICK C ,  ARNOLD FRANCES H

IPC: C07H21/04 ,  C12N9/02 ,  C12Q1/68 ,  C12N15/00

CPC classification number: C12N9/0071 ,  C12Y114/14001

Abstract: The invention relates to novel variants of cytochrome P450 oxygenases. These variants have an improved ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. These variants also have a improved thermostability as compared to the cytochrome P450 BM-3 F87A mutant. Preferred variants include cytochrome P450 BM-3 heme domain mutants having I58V, F87A, H100R, F107L, A135S, M145A/V, N239H, S274T. L324I, I366V, K434E, E442K, and/or V446I amino acid substitutions.

公开(公告)号：EP1660646A4

公开(公告)日：2008-01-02

申请号：EP04781001

申请日：2004-08-11

Applicant: CALIFORNIA INST OF TECHN

Inventor： ARNOLD FRANCES H ,  CIRINO PATRICK C

IPC: C12N9/02 ,  C07H21/04 ,  C12N20060101 ,  C12N5/06 ,  C12N15/53 ,  C12P21/02

CPC classification number: C12N9/0077

Abstract: The invention relates to novel variants of cytochrome P450 oxygenases. These variants have at least one mutation improving their ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. The variants also have at least one mutation improving thermostability as compared to the parent enzyme or corresponding wild-type enzyme. Preferred variants include cytochrome P450 BM-3 heme domain variants having L52I, I58V, F87A, H100R, S106R, F107L, A135S, M145A/V, A184V, N239H, S274T, L324I, V340M, I366V, K434E, E442K, and/or V446I amino acid substitutions.

公开(公告)号：AU2002307340A1

公开(公告)日：2002-10-28

申请号：AU2002307340

申请日：2002-04-16

Applicant: CALIFORNIA INST OF TECHN

Inventor： ARNOLD FRANCES H ,  CIRINO PATRICK C

IPC: C07H21/04 ,  C12N9/02 ,  C12Q1/68 ,  C12N15/00

公开(公告)号：DK1660646T3

公开(公告)日：2015-03-09

申请号：DK04781001

申请日：2004-08-11

Applicant: CALIFORNIA INST OF TECHN

Inventor： CIRINO PATRICK C ,  ARNOLD FRANCES H

IPC: C12N15/53 ,  C07H21/04 ,  C12N20060101 ,  C12N5/06 ,  C12N9/02 ,  C12P21/02