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公开(公告)号：WO9961888A8
公开(公告)日：2000-04-20
申请号：PCT/US9913050
申请日：1999-05-21
Applicant: CALIFORNIA INST OF TECHN , QUAKE STEPHEN , FU ANNE , ARNOLD FRANCES , SPENCE CHARLES F
Inventor： QUAKE STEPHEN , FU ANNE , ARNOLD FRANCES , SPENCE CHARLES F
IPC: G01N21/64 , C12Q1/04 , G01N15/14 , G01N21/05 , G01N21/33 , G01N21/78 , G01N27/447 , G01N33/48 , G01N33/58 , G01N33/60 , G01N37/00
CPC classification number: G01N15/14 , G01N2015/149
Abstract: The invention provides a microfabricated device for sorting cells based on a desired characteristic, for example, reporter-labeled cells can be sorted by the presence or level of reporter on the cells. The device includes a chip having a substrate into which is microfabricated at least one analysis unit. Each analysis unit includes a main channel, having a sample inlet channel, typically at one end, and a detection region along a portion of its length. Adjacent and downstream from the detection region, the main channel has a discrimination region or branch point leading to at least two branch channels. The analysis unit may further include additional inlet channels, detection points, branch points, and branch channels as desired. A stream containing cells is passed through the detection region, such that on average one cell occupies the detection region at a given time. The cells can be sorted into an appropriate branch channel based on the presence or amount of a detectable signal such as an optical signal, with or without stimulation, such as exposure to light in order to promote fluorescence.
Abstract translation: 本发明提供了用于基于期望的特征来分选细胞的微制造装置，例如，可以通过细胞上报道分子的存在或水平来分选报道分子标记的细胞。 该装置包括具有基板的芯片，微制造至少一个分析单元。 每个分析单元包括主通道，其具有典型地在一端处的样本入口通道以及沿其长度的一部分的检测区域。 在检测区域的附近和下游，主信道具有通向至少两个分支信道的鉴别区域或分支点。 分析单元可根据需要进一步包括附加入口通道，检测点，分支点和分支通道。 包含细胞的流通过检测区域，使得平均一个细胞在给定时间占据检测区域。 基于可检测信号（诸如光学信号）的存在或量，在有或没有刺激（例如暴露于光以促进荧光）的情况下，可以将细胞分选到合适的分支通道中。
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公开(公告)号：WO9961888A2
公开(公告)日：1999-12-02
申请号：PCT/US9913050
申请日：1999-05-21
Applicant: CALIFORNIA INST OF TECHN , QUAKE STEPHEN , FU ANNE , ARNOLD FRANCES , SPENCE CHARLES F
Inventor： QUAKE STEPHEN , FU ANNE , ARNOLD FRANCES , SPENCE CHARLES F
IPC: G01N21/64 , C12Q1/04 , G01N15/14 , G01N21/05 , G01N21/33 , G01N21/78 , G01N27/447 , G01N33/48 , G01N33/58 , G01N33/60 , G01N37/00
CPC classification number: G01N15/14 , G01N2015/149
Abstract: The invention provides a microfabricated device for sorting cells based on a desired characteristic, for example, reporter-labeled cells can be sorted by the presence or level of reporter on the cells. The device includes a chip having a substrate into which is microfabricated at least one analysis unit. Each analysis unit includes a main channel, having a sample inlet channel, typically at one end, and a detection region along a portion of its length. Adjacent and downstream from the detection region, the main channel has a discrimination region or branch point leading to at least two branch channels. The analysis unit may further include additional inlet channels, detection points, branch points, and branch channels as desired. A stream containing cells is passed through the detection region, such that on average one cell occupies the detection region at a given time. The cells can be sorted into an appropriate branch channel based on the presence or amount of a detectable signal such as an optical signal, with or without stimulation, such as exposure to light in order to promote fluorescence.
Abstract translation: 本发明提供了一种用于基于所需特征分选细胞的微制造装置，例如，报道分子标记的细胞可以通过细胞上报告物的存在或水平进行分选。 该装置包括具有基板的芯片，微基板将至少一个分析单元微加工。 每个分析单元包括主通道，具有通常在一端的样品入口通道和沿其长度的一部分的检测区域。 相邻和下游的检测区域，主通道具有通向至少两个分支通道的识别区域或分支点。 分析单元还可以根据需要进一步包括额外的入口通道，检测点，分支点和分支通道。 包含细胞的流通过检测区域，使得平均一个细胞在给定时间占据检测区域。 基于可检测信号（例如光信号）的存在或量，可以将细胞分类到适当的分支通道中，具有或不具有刺激，例如暴露于光以促进荧光。
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Patent Agency Ranking
公开(公告)号：WO9961888A9
公开(公告)日：2000-06-02
申请号：PCT/US9913050
申请日：1999-05-21
Applicant: CALIFORNIA INST OF TECHN , QUAKE STEPHEN , FU ANNE , ARNOLD FRANCES , SPENCE CHARLES F
Inventor： QUAKE STEPHEN , FU ANNE , ARNOLD FRANCES , SPENCE CHARLES F
IPC: G01N21/64 , C12Q1/04 , G01N15/14 , G01N21/05 , G01N21/33 , G01N21/78 , G01N27/447 , G01N33/48 , G01N33/58 , G01N33/60 , G01N37/00
CPC classification number: G01N15/14 , G01N2015/149
Abstract: The invention provides a microfabricated device for sorting cells based on a desired characteristic, for example, reporter-labeled cells can be sorted by the presence or level of reporter on the cells. The device includes a chip having a substrate into which is microfabricated at least one analysis unit. Each analysis unit includes a main channel, having a sample inlet channel, typically at one end, and a detection region along a portion of its length. Adjacent and downstream from the detection region, the main channel has a discrimination region or branch point leading to at least two branch channels. The analysis unit may further include additional inlet channels, detection points, branch points, and branch channels as desired. A stream containing cells is passed through the detection region, such that on average one cell occupies the detection region at a given time. The cells can be sorted into an appropriate branch channel based on the presence or amount of a detectable signal such as an optical signal, with or without stimulation, such as exposure to light in order to promote fluorescence.
Abstract translation: 本发明提供了一种用于基于所需特征分选细胞的微制造装置，例如，报道分子标记的细胞可以通过细胞上报告物的存在或水平进行分选。 该装置包括具有基板的芯片，微基板将至少一个分析单元微加工。 每个分析单元包括主通道，具有通常在一端的样品入口通道和沿其长度的一部分的检测区域。 相邻和下游的检测区域，主通道具有通向至少两个分支通道的识别区域或分支点。 分析单元还可以根据需要进一步包括额外的入口通道，检测点，分支点和分支通道。 包含细胞的流通过检测区域，使得平均一个细胞在给定时间占据检测区域。 基于可检测信号（例如光信号）的存在或量，可以将细胞分类到适当的分支通道中，具有或不具有刺激，例如暴露于光以促进荧光。
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公开(公告)号：CA2333201A1
公开(公告)日：1999-12-02
申请号：CA2333201
申请日：1999-05-21
Applicant: CALIFORNIA INST OF TECHN
Inventor： QUAKE STEPHEN , FU ANNE , SPENCE CHARLES F , ARNOLD FRANCES
IPC: G01N21/64 , C12Q1/04 , G01N15/14 , G01N21/05 , G01N21/33 , G01N21/78 , G01N27/447 , G01N33/48 , G01N33/58 , G01N33/60 , G01N37/00
Abstract: The invention provides a microfabricated device for sorting cells based on a desired characteristic, for example, reporter-labeled cells can be sorted by the presence or level of reporter on the cells. The device includes a chip having a substrate into which is microfabricated at least one analysis unit. Each analysis unit includes a main channel, having a sample inlet channel, typically at one end, and a detection region along a portion of its length. Adjacent and downstream from the detection region, the main channel has a discrimination region or branch point leading to at least two branch channel s. The analysis unit may further include additional inlet channels, detection points, branch points, and branch channels as desired. A stream containing cells is passed through the detection region, such that on average one cell occupies the detection region at a given time. The cells can be sorted into an appropriate branch channel based on the presence or amount of a detectable signal such as an optical signal, with or without stimulation, such as exposure to light in order to promote fluorescence.
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公开(公告)号：AU4955799A
公开(公告)日：1999-12-13
申请号：AU4955799
申请日：1999-05-21
Applicant: CALIFORNIA INST OF TECHN
Inventor： QUAKE STEPHEN , FU ANNE , ARNOLD FRANCES , SPENCE CHARLES F
IPC: G01N21/64 , C12Q1/04 , G01N15/14 , G01N21/05 , G01N21/33 , G01N21/78 , G01N27/447 , G01N33/48 , G01N33/58 , G01N33/60 , G01N37/00
Abstract: The invention provides a microfabricated device for sorting cells based on a desired characteristic, for example, reporter-labeled cells can be sorted by the presence or level of reporter on the cells. The device includes a chip having a substrate into which is microfabricated at least one analysis unit. Each analysis unit includes a main channel, having a sample inlet channel, typically at one end, and a detection region along a portion of its length. Adjacent and downstream from the detection region, the main channel has a discrimination region or branch point leading to at least two branch channels. The analysis unit may further include additional inlet channels, detection points, branch points, and branch channels as desired. A stream containing cells is passed through the detection region, such that on average one cell occupies the detection region at a given time. The cells can be sorted into an appropriate branch channel based on the presence or amount of a detectable signal such as an optical signal, with or without stimulation, such as exposure to light in order to promote fluorescence.
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公开(公告)号：AT530891T
公开(公告)日：2011-11-15
申请号：AT99933512
申请日：1999-05-21
Applicant: CALIFORNIA INST OF TECHN
Inventor： QUAKE STEPHEN , FU ANNE , ARNOLD FRANCES , SPENCE CHARLES
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公开(公告)号：AU763433B2
公开(公告)日：2003-07-24
申请号：AU4955799
申请日：1999-05-21
Applicant: CALIFORNIA INST OF TECHN
Inventor： QUAKE STEPHEN , FU ANNE , ARNOLD FRANCES , SPENCE CHARLES F
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