公开(公告)号：US11969246B2

公开(公告)日：2024-04-30

申请号：US18319085

申请日：2023-05-17

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Samson Chen ,  Axel Scherer ,  Dvin I. Adalian ,  Peter Petillo ,  Muhammad Musab Jilani ,  Xiomara L. Madero ,  Deepan Kishore Kumar

IPC: C12N11/14 ,  A61B5/145 ,  A61B5/1459 ,  A61B5/1486 ,  C12N9/02 ,  C12N11/02 ,  G01N27/327

CPC classification number: A61B5/14865 ,  A61B5/14532 ,  A61B5/1459 ,  C12N9/0004 ,  C12N11/02 ,  C12N11/14 ,  G01N27/3272 ,  C12Y101/01001 ,  C12Y101/03003 ,  C12Y101/03005 ,  C12Y101/03007 ,  C12Y101/03008 ,  C12Y101/0301 ,  C12Y101/03011 ,  C12Y101/03012 ,  C12Y101/03015 ,  C12Y101/03016 ,  C12Y101/03018 ,  C12Y101/03019 ,  C12Y101/0302 ,  C12Y101/03023 ,  C12Y101/03027 ,  C12Y101/03029 ,  C12Y101/0303 ,  C12Y101/03037 ,  C12Y101/03038 ,  C12Y101/0304 ,  C12Y101/03041

Abstract: A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.