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公开(公告)号:US11969246B2
公开(公告)日:2024-04-30
申请号:US18319085
申请日:2023-05-17
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor: Samson Chen , Axel Scherer , Dvin I. Adalian , Peter Petillo , Muhammad Musab Jilani , Xiomara L. Madero , Deepan Kishore Kumar
IPC: C12N11/14 , A61B5/145 , A61B5/1459 , A61B5/1486 , C12N9/02 , C12N11/02 , G01N27/327
CPC classification number: A61B5/14865 , A61B5/14532 , A61B5/1459 , C12N9/0004 , C12N11/02 , C12N11/14 , G01N27/3272 , C12Y101/01001 , C12Y101/03003 , C12Y101/03005 , C12Y101/03007 , C12Y101/03008 , C12Y101/0301 , C12Y101/03011 , C12Y101/03012 , C12Y101/03015 , C12Y101/03016 , C12Y101/03018 , C12Y101/03019 , C12Y101/0302 , C12Y101/03023 , C12Y101/03027 , C12Y101/03029 , C12Y101/0303 , C12Y101/03037 , C12Y101/03038 , C12Y101/0304 , C12Y101/03041
Abstract: A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.