公开(公告)号：US11427865B2

公开(公告)日：2022-08-30

申请号：US16927496

申请日：2020-07-13

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F Ismagilov ,  Jacob T Barlow ,  Said R. Bogatyrev

IPC: C12Q1/68 ,  C12Q1/686 ,  C12Q1/6888

Abstract: Provided herein are methods and systems for absolute quantification of a target 16S rRNA and/or of a target prokaryotic taxon, based on amplifying and sequencing a same 16S rRNA recognition segment in which target 16S rRNA conserved regions flank 16S rRNA variable regions, conserved and variable among a plurality of sample 16S rRNAs and/or of a sample prokaryotic taxon of higher taxonomic rank with respect to the target taxon. In the methods and systems, absolute abundance of the a plurality of sample 16S rRNAs and/or of the sample prokaryotic taxon detected by the amplifying, is multiplied by the relative abundance of the target 16S rRNA and/or of a target prokaryotic taxon detected by the sequencing to provide the absolute quantification in accordance with method and systems of the disclosure.

公开(公告)号：US12173363B2

公开(公告)日：2024-12-24

申请号：US17863183

申请日：2022-07-12

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. Ismagilov ,  Jacob T. Barlow ,  Said R. Bogatyrev

IPC: C12Q1/68 ,  C12Q1/686 ,  C12Q1/6888

Abstract: Provided herein are methods and systems for absolute quantification of a target 16S rRNA and/or of a target prokaryotic taxon, based on amplifying and sequencing a same 16S rRNA recognition segment in which target 16S rRNA conserved regions flank 16S rRNA variable regions, conserved and variable among a plurality of sample 16S rRNAs and/or of a sample prokaryotic taxon of higher taxonomic rank with respect to the target taxon. In the methods and systems, absolute abundance of the a plurality of sample 16S rRNAs and/or of the sample prokaryotic taxon detected by the amplifying, is multiplied by the relative abundance of the target 16S rRNA and/or of a target prokaryotic taxon detected by the sequencing to provide the absolute quantification in accordance with method and systems of the disclosure.