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公开(公告)号：NO20061576A
公开(公告)日：2006-04-07
申请号：NO20061576
申请日：2006-04-07
Applicant: CELLTECH R&D LTD
Inventor： SEHDEV MUKESH , REEKS DOMINIC GAMBIER , GLOVER DAVID JOHN
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Patent Agency Ranking
公开(公告)号：CA2536099A1
公开(公告)日：2005-03-17
申请号：CA2536099
申请日：2004-09-02
Applicant: CELLTECH R&D LTD
Inventor： GLOVER DAVID JOHN , SEHDEV MUKESH , REEKS DOMINIC GAMBIER
Abstract: The present invention provides a method for controlling the partitioning of a recombinant protein between the supernatant and the periplasm in E. coli host cell cultures wherein expression of the recombinant protein by said cells is under the control of an inducible system, which method comprises: a) providing an E. coli host cell culture b) changing the growth rate of the E. coli host cells c) inducing expression of the recombinant protein wherein steps (b) and (c) can be performed in any order or simultaneously; and subsequently d) determining the yield of recombinant protein in the culture supernatant and the E. coli host cell periplasm e) comparing the yield determined in step (d) with the yield determined when at least one other growth rate has been used in step (b) f) selecting a growth rate from the comparison made in step (e) in which the partitioning of the recombinant protein between the supernatant and the periplasm is most suited to the primary recovery of the recombinant protein.
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公开(公告)号：BRPI0413813A
公开(公告)日：2006-10-17
申请号：BRPI0413813
申请日：2004-09-02
Applicant: CELLTECH R&D LTD
Inventor： GLOVER DAVID JOHN , SEHDEV MUKESH , REEKS DOMINIC GAMBIER
Abstract: The present invention provides a method for controlling the partitioning of a recombinant protein between the supernatant and the periplasm in E. coli host cell cultures wherein expression of the recombinant protein by said cells is under the control of an inducible system, which method comprises: a) providing an E. coli host cell culture b) changing the growth rate of the E. coli host cells c) inducing expression of the recombinant protein wherein steps (b) and (c) can be performed in any order or simultaneously; and subsequently d) determining the yield of recombinant protein in the culture supernatant and the E. coli host cell periplasm e) comparing the yield determined in step (d) with the yield determined when at least one other growth rate has been used in step (b) f) selecting a growth rate from the comparison made in step (e) in which the partitioning of the recombinant protein between the supernatant and the periplasm is most suited to the primary recovery of the recombinant protein.
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公开(公告)号：CA2536099C
公开(公告)日：2015-11-17
申请号：CA2536099
申请日：2004-09-02
Applicant: CELLTECH R&D LTD
Inventor： GLOVER DAVID JOHN , SEHDEV MUKESH , REEKS DOMINIC GAMBIER
Abstract: The present invention provides a method for controlling the partitioning of a recombinant protein between the supernatant and the periplasm in E.coli host cell cultures wherein expression of the recombinant protein by said cells is under the control of an inducible system, which method comprises: a) providing an E.coli host cell culture b) changing the growth rate of theE.coli host cells c) inducing expression of the recombinant protein wherein steps (b) and (c) can be performed in any order or simultaneously; and subsequently d) determining the yield of recombinant protein in the culture supernatant and the E.coli host cell periplasm e) comparing the yield determined in step (d) with the yield determined when at least one other growth rate has been used in step (b) f) selecting a growth rate from the comparison made in step (e) in which the partitioning of the recombinant protein between the supernatant and the periplasm is most suited to the primary recovery of the recombinant protein.
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公开(公告)号：NO20061576L
公开(公告)日：2006-04-07
申请号：NO20061576
申请日：2006-04-07
Applicant: CELLTECH R&D LTD
Inventor： SEHDEV MUKESH , REEKS DOMINIC GAMBIER , GLOVER DAVID JOHN
Abstract: The present invention provides a method for controlling the partitioning of a recombinant protein between the supernatant and the periplasm in E. coli host cell cultures wherein expression of the recombinant protein by said cells is under the control of an inducible system, which method comprises: a) providing an E. coli host cell culture b) changing the growth rate of the E. coli host cells c) inducing expression of the recombinant protein wherein steps (b) and (c) can be performed in any order or simultaneously; and subsequently d) determining the yield of recombinant protein in the culture supernatant and the E. coli host cell periplasm e) comparing the yield determined in step (d) with the yield determined when at least one other growth rate has been used in step (b) f) selecting a growth rate from the comparison made in step (e) in which the partitioning of the recombinant protein between the supernatant and the periplasm is most suited to the primary recovery of the recombinant protein.
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公开(公告)号：AU2004270929A1
公开(公告)日：2005-03-17
申请号：AU2004270929
申请日：2004-09-02
Applicant: CELLTECH R&D LTD
Inventor： REEKS DOMINIC GAMBIER , SEHDEV MUKESH , GLOVER DAVID JOHN
Abstract: The present invention provides a method for controlling the partitioning of a recombinant protein between the supernatant and the periplasm in E. coli host cell cultures wherein expression of the recombinant protein by said cells is under the control of an inducible system, which method comprises: a) providing an E. coli host cell culture b) changing the growth rate of the E. coli host cells c) inducing expression of the recombinant protein wherein steps (b) and (c) can be performed in any order or simultaneously; and subsequently d) determining the yield of recombinant protein in the culture supernatant and the E. coli host cell periplasm e) comparing the yield determined in step (d) with the yield determined when at least one other growth rate has been used in step (b) f) selecting a growth rate from the comparison made in step (e) in which the partitioning of the recombinant protein between the supernatant and the periplasm is most suited to the primary recovery of the recombinant protein.
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