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公开(公告)号：WO2007105041A2
公开(公告)日：2007-09-20
申请号：PCT/IB2007000136
申请日：2007-01-22
Applicant: COUNCIL SCIENT IND RES , PUROHIT HEMANT JYOTISWARUP , KAPLEY ATYA , RAJE DHANANJAY VASANT , DEVOTTA SUKUMAR
Inventor： PUROHIT HEMANT JYOTISWARUP , KAPLEY ATYA , RAJE DHANANJAY VASANT , DEVOTTA SUKUMAR
IPC: C12Q1/68
CPC classification number: C12Q1/689
Abstract: Present invention relates to a method to determine the genotype of organisms by RAPD analysis and more specifically, to establish the relatedness of individual organisms across and within species. RAPD uses genotypic information of an organism to give an organism specific DNA fragment of different sizes. The present invention provide methods and a set of oligonucleotide primers for performing amplification and other enzymatic reactions on nucleic acid molecules that have been collected directly as environmental DNA or DNA derived form pure isolates. More specifically, the present invention relates to a novel method of genetic analysis using a set of sub-sequence, which occurs as inverted repeats in different genome with different frequencies. All bacterial cultures used in this study have been isolated from activated biomass collected from effluent treatment plants. The bacteria have been sub-cultured repeatedly to obtain pure cultures. All plating has been carried out on Luria Broth plates with 2% agar. The 16S rRNA gene has been amplified using universal primers to confirm the eubacterial nature of the isolates. The primers used to amplify a 1466-bp product were 27F forward primer 5'- AGAGTTTGATCMTGGCTCAG-3' and 1492 reverse primer 5'- TACGGYTACCTTGTTACGACTT-Hence, in defined conditions two genome samples could be differentiated from each other. These features are applicable to DNA fingerprinting, marker assisted selection, genotyping, and high throughput laboratory screening methods for culturable microbes from any environmental niche.
Abstract translation: 本发明涉及通过RAPD分析确定生物体基因型的方法，更具体地说，用于建立物种间和物种内个体生物体的相关性。 RAPD使用生物体的基因型信息给出不同大小的生物体特异性DNA片段。 本发明提供了用于对已经直接作为环境DNA或源自纯分离物的DNA收集的核酸分子进行扩增和其他酶促反应的方法和一组寡核苷酸引物。 更具体地，本发明涉及一种使用一组子序列的遗传分析的新方法，其以不同基因组的不同频率的反向重复出现。 本研究中使用的所有细菌培养物都是从污水处理厂收集的活性生物质中分离出来的。 细菌已经被重复培养以获得纯培养物。 在含有2％琼脂的Luria肉汤培养板上进行所有的电镀。 使用通用引物扩增16S rRNA基因以确认分离株的真细菌性质。 用于扩增1466bp产物的引物是27F正向引物5'-AGAGTTTGATCMTGGCTCAG-3'和1492反向引物5'-TACGGYTACCTTGTTACGACTT-因此，在确定的条件下，两种基因组样品可以相互区分。 这些功能适用于DNA指纹识别，标记辅助选择，基因分型和高通量实验室筛选方法，可用于任何环境生态位的可培养微生物。
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公开(公告)号：AU2007330354A1
公开(公告)日：2008-06-12
申请号：AU2007330354
申请日：2007-12-05
Applicant: COUNCIL SCIENT IND RES
Inventor： DEVOTTA SUKUMAR , NANDY TAPAS , KAUL SANTOSH NARAIN , POPHALI GIRISH RAMESH
IPC: B01D21/00
Abstract: The improved circular secondary clarifier of the present invention requires less surface area and low HRT and provides efficient solids-liquid separation. The improved clarifier has better SS and BOD reduction and provides high under flow solids concentrations, as compared to conventional secondary clarifiers. The improved clarifier is even capable of treating wastewaters containing low MLSS concentration. One of the biggest advantages of improved clarifier is that it does not require a separate sump cum pump house as it removes the settled sludge using suction mechanism thereby reduces capital and recurring cost. Further, it provides natural flocculation, which is essential for agglomeration of solids and increases particle size referred to as 'floc' and eliminates provision for a separate flocculation facility thereby reduces capital and recurring costs.
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公开(公告)号：AU2007330354B2
公开(公告)日：2012-06-28
申请号：AU2007330354
申请日：2007-12-05
Applicant: COUNCIL SCIENT IND RES
Inventor： KAUL SANTOSH NARAIN , POPHALI GIRISH RAMESH , NANDY TAPAS , DEVOTTA SUKUMAR
IPC: B01D21/00
Abstract: The improved circular secondary clarifier of the present invention requires less surface area and low HRT and provides efficient solids-liquid separation. The improved clarifier has better SS and BOD reduction and provides high under flow solids concentrations, as compared to conventional secondary clarifiers. The improved clarifier is even capable of treating wastewaters containing low MLSS concentration. One of the biggest advantages of improved clarifier is that it does not require a separate sump cum pump house as it removes the settled sludge using suction mechanism thereby reduces capital and recurring cost. Further, it provides natural flocculation, which is essential for agglomeration of solids and increases particle size referred to as 'floc' and eliminates provision for a separate flocculation facility thereby reduces capital and recurring costs.
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公开(公告)号：AU2003304661A1
公开(公告)日：2005-07-21
申请号：AU2003304661
申请日：2003-12-31
Applicant: COUNCIL SCIENT IND RES
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