公开(公告)号：US09624487B2

公开(公告)日：2017-04-18

申请号：US14069697

申请日：2013-11-01

Applicant: California Institute of Technology

Inventor： Samuel Njoroge ,  George Maltezos ,  Axel Scherer ,  John B. Gorman

IPC: C12N15/10 ,  B01D15/22

CPC classification number: C12N15/101 ,  B01D15/22

Abstract: An apparatus for purifying nucleic acid can include a disposable cartridge having a layered configuration, and a disposable syringe for coupling to the disposable cartridge such that a fluid can be delivered to or withdrawn fluid from the disposable cartridge using the syringe. At least one layer of the disposable cartridge can include a cut-out for forming a functional unit of the disposable cartridge, and at least one layer of the disposable cartridge can be joined to an adjacent layer of the disposable cartridge by an adhesive material layer. The functional unit can include a fluid channel between an access port on the first surface of the disposable cartridge and the fluid pouch. The apparatus can include a disposable silica-containing material for binding with the nucleic acid. The apparatus can include a heating element and a temperature sensor for heating the disposable silica-containing material.

公开(公告)号：US20140134078A1

公开(公告)日：2014-05-15

申请号：US14069697

申请日：2013-11-01

Applicant: California Institute of Technology

Inventor： Samuel Njoroge ,  George Maltezos ,  Axel Scherer ,  John B. Gorman

IPC: C12N15/10

CPC classification number: C12N15/101 ,  B01D15/22

Abstract: An apparatus for purifying nucleic acid can include a disposable cartridge having a layered configuration, and a disposable syringe for coupling to the disposable cartridge such that a fluid can be delivered to or withdrawn fluid from the disposable cartridge using the syringe. At least one layer of the disposable cartridge can include a cut-out for forming a functional unit of the disposable cartridge, and at least one layer of the disposable cartridge can be joined to an adjacent layer of the disposable cartridge by an adhesive material layer. The functional unit can include a fluid channel between an access port on the first surface of the disposable cartridge and the fluid pouch. The apparatus can include a disposable silica-containing material for binding with the nucleic acid. The apparatus can include a heating element and a temperature sensor for heating the disposable silica-containing material.

Abstract translation: 用于纯化核酸的装置可以包括具有分层构造的一次性盒，以及用于连接到一次性盒的一次性注射器，使得可以使用注射器将流体从一次性盒输送到或从其中抽出流体。 一次性盒的至少一层可以包括用于形成一次性盒的功能单元的切口，并且一次性盒的至少一层可以通过粘合材料层连接到一次性盒的相邻层。 功能单元可以包括在一次性盒的第一表面上的进入端口和流体袋之间的流体通道。 该装置可以包括用于与核酸结合的一次性含二氧化硅的材料。 该装置可以包括用于加热一次性二氧化硅的材料的加热元件和温度传感器。

公开(公告)号：US09580679B2

公开(公告)日：2017-02-28

申请号：US14033365

申请日：2013-09-20

Applicant: California Institute of Technology

Inventor： Samuel Njoroge ,  John Gorman ,  George Maltezos ,  Axel Scherer

IPC: C12N1/06 ,  B01L3/00 ,  B01L7/00 ,  C12M1/00 ,  B01J19/24 ,  G01N27/447

CPC classification number: C12M47/06 ,  B01L3/5027 ,  B01L7/52 ,  B01L2300/1883 ,  C12M21/00 ,  C12N1/06 ,  C12N1/066 ,  G01N27/44791

Abstract: Disclosed herein are methods and systems for use in preparing a sample. The methods and systems may be used for lysing one or more structures in a sample (e.g., cells, viral particles, etc.). The methods and compositions may comprise a microfluidic chip or use thereof. The microfluidic chips disclosed herein may comprise (a) a substrate comprising a chamber, wherein at least one mechanical element may be located within the chamber; (b) a thermal element in contact with the chamber; and (c) at least one aperture within the surface of the substrate, wherein the aperture may be configured to insulate the chamber.

Abstract translation: 本文公开了用于制备样品的方法和系统。 方法和系统可用于裂解样品中的一种或多种结构（例如，细胞，病毒颗粒等）。 所述方法和组合物可以包含微流控芯片或其用途。 本文公开的微流体芯片可以包括（a）包括腔室的基底，其中至少一个机械元件可以位于腔室内; （b）与所述室接触的热元件; 以及（c）在所述基底表面内的至少一个孔，其中所述孔可以被配置为使所述腔绝缘。

公开(公告)号：US20140073013A1

公开(公告)日：2014-03-13

申请号：US13961752

申请日：2013-08-07

Applicant: California Institute of Technology

Inventor： John B. GORMAN ,  George Maltezos ,  Axel Scherer

IPC: B01L7/00

CPC classification number: B01L7/52 ,  B01L2200/025 ,  B01L2300/0816 ,  B01L2300/1827 ,  B01L2300/1844

Abstract: This disclosure provides thermal cyclers, systems, and methods of thermally cycling a sample.

Abstract translation: 本公开提供热循环仪，系统和热循环样品的方法。