公开(公告)号：US11624062B2

公开(公告)日：2023-04-11

申请号：US16141901

申请日：2018-09-25

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. Ismagilov ,  Matthew S. Curtis ,  Mary Arrastia ,  David A. Selck ,  Mitchell Guttman

IPC: C12N15/10 ,  B01L3/00 ,  C12Q1/6855 ,  G01N33/58 ,  G01N33/68 ,  C12Q1/6806 ,  C40B30/04 ,  C40B40/08 ,  C40B70/00

Abstract: Methods, systems and related compositions are provided to perform single-cell marking of a nucleic acid and/or protein in a sample based on in-cell or in-organelle barcoding of nucleic acid and/or protein complexes of the cell or organelle; the methods and systems herein described are configured to provide in-cell or in-organelle single-cell marked nucleic acid and/or protein complexes comprising a single-cell, cell-specific, or a single-cell organelle-specific marker.

公开(公告)号：US11249071B2

公开(公告)日：2022-02-15

申请号：US15138208

申请日：2016-04-25

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Mitchell Guttman ,  Chun-Kan Chen ,  Colleen A. McHugh

IPC: G01N33/50

Abstract: Methods of prohibiting Xist-dependent silencing of X chromosome genes include targeting the required Xist silencing complex components including SHARP, SMRT, HDAC3, SAF-A, LBR, and the respective binding sites of SHARP, LBR, and SAF-A on Xist, thereby prohibiting Xist repression, allowing for reactivation of the silenced X chromosome genes.

公开(公告)号：US20200088729A1

公开(公告)日：2020-03-19

申请号：US16573673

申请日：2019-09-17

Applicant: California Institute of Technology

Inventor： Mitchell Guttman ,  Mario R. Blanco

IPC: G01N33/573 ,  C12N15/90

Abstract: In some embodiments, methods of detecting an association between a query protein and a target moiety are described. In some embodiments, compositions are described. In some embodiments, kits are described.

公开(公告)号：US20160313304A1

公开(公告)日：2016-10-27

申请号：US15138208

申请日：2016-04-25

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Mitchell Guttman ,  Chun-Kan Chen ,  Colleen A. McHugh

IPC: G01N33/50

CPC classification number: G01N33/5023

Abstract: Methods of prohibiting Xist-dependent silencing of X chromosome genes include targeting the required Xist silencing complex components including SHARP, SMRT, HDAC3, SAF-A, LBR, and the respective binding sites of SHARP, LBR, and SAF-A on Xist, thereby prohibiting Xist repression, allowing for reactivation of the silenced X chromosome genes.

Abstract translation: 禁止X染色体基因Xist依赖性沉默的方法包括靶向所需的Xist沉默复合物成分，包括SHARP，SMRT，HDAC3，SAF-A，LBR，以及Xist上SHARP，LBR和SAF-A的相应结合位点，从而 禁止Xist镇压，允许重新激活沉默的X染色体基因。

公开(公告)号：US11078480B2

公开(公告)日：2021-08-03

申请号：US16575124

申请日：2019-09-18

Applicant: California Institute of Technology

Inventor： Mitchell Guttman ,  Sofia A. Quinodoz ,  Mario R. Blanco ,  Devdoot Majumdar

IPC: C12N15/10 ,  G01N33/53 ,  G01N33/68

Abstract: Methods of detecting an interaction between a macromolecule and an interaction partner are described. Kits are also described.

公开(公告)号：US10900974B2

公开(公告)日：2021-01-26

申请号：US15466861

申请日：2017-03-22

Applicant: California Institute of Technology

Inventor： Sofia A. Quinodoz ,  Mitchell Guttman

IPC: G01N33/68 ,  C12N15/10 ,  C12Q1/6806 ,  G01N33/58

Abstract: A method for identifying interactions of DNA, RNA, and/or protein molecules in a cell includes distributing a cell lysate or fraction thereof into a plurality of lysate suspensions, adding a unique nucleotide tag to each lysate suspension to tag each DNA, RNA, and/or protein, pooling the tagged suspensions, and repeating the tagging, pooling, and sorting (distributing) as desired to decrease the probability that non-interacting molecules will receive all of the same nucleotide tags.

公开(公告)号：US20240384325A1

公开(公告)日：2024-11-21

申请号：US18665401

申请日：2024-05-15

Applicant: California Institute of Technology ,  The Trustees of Columbia University in the City of New York

Inventor： Erica Wolin ,  James Guo ,  Mitchell Guttman ,  Marko Jovanovic

IPC: C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6869

Abstract: Aspects of the present disclosure generally relate to methods and compositions for detecting an association between a RNA binding protein and a RNA. Some aspects of the present disclosure relate to methods of generating an antibody-bead conjugate pool. Some aspects of the present disclosure relate to kits and compositions for performing the methods disclosed herein.

公开(公告)号：US11197881B2

公开(公告)日：2021-12-14

申请号：US16345666

申请日：2017-10-27

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Mitchell Guttman ,  Chun-Kan Chen

IPC: A61K31/706 ,  A61K31/165 ,  A61K31/405 ,  A61K31/473 ,  C12N15/01

Abstract: A reactivation composition for activating or re-activating expression of a silenced X chromosome gene in a cell includes a non-cytotoxic histone deacetylase (HDAC) inhibitor. The reactivation composition includes the non-cytotoxic HDAC inhibitor and may further include a DNA methylation inhibitor. A method of activating or re-activating expression of a silenced X chromosome gene in a cell includes administering a reactivation composition including a non-cytotoxic HDAC inhibitor. The method of activating or re-activating expression of a silenced X chromosome gene may further includes administering a reactivation composition that includes a non-cytotoxic HDAC inhibitor and an inhibitor of DNA methylation.

公开(公告)号：US20200087657A1

公开(公告)日：2020-03-19

申请号：US16575124

申请日：2019-09-18

Applicant: California Institute of Technology

Inventor： Mitchell Guttman ,  Sofia A. Quinodoz ,  Mario R. Blanco ,  Devdoot Majumdar

IPC: C12N15/10 ,  G01N33/68 ,  G01N33/53

Abstract: Methods of detecting an interaction between a macromolecule and an interaction partner are described. Kits are also described.

公开(公告)号：US20190187156A1

公开(公告)日：2019-06-20

申请号：US15466861

申请日：2017-03-22

Applicant: California Institute of Technology

Inventor： Sofia A. Quinodoz ,  Mitchell Guttman

IPC: G01N33/68 ,  C12N15/10

CPC classification number: G01N33/6875 ,  C12N15/1065

Abstract: A method for identifying interactions of DNA, RNA, and/or protein molecules in a cell includes distributing a cell lysate or fraction thereof into a plurality of lysate suspensions, adding a unique nucleotide tag to each lysate suspension to tag each DNA, RNA, and/or protein, pooling the tagged suspensions, and repeating the tagging, pooling, and sorting (distributing) as desired to decrease the probability that non-interacting molecules will receive all of the same nucleotide tags.