公开(公告)号：EP0040466A3

公开(公告)日：1982-12-08

申请号：EP81301304

申请日：1981-03-26

Applicant: Canadian Patents and Development Limited

Inventor： Narang, Saran A. ,  Wu, Ray J.

IPC: C12N15/00 ,  C07H21/04 ,  C12P21/02 ,  C12N01/20 ,  C12R01/19

CPC classification number: C12N15/10 ,  C07K14/62 ,  C12N15/66 ,  C12N15/70

Abstract: Adaptor molecules have been prepared to comprise either start or stop signals for protein synthesis, in addition to recognition sites for restriction endonucleases. Separate adaptors may be used in a symmetrical duplex form. The start adaptor may include nucleotide base inserts to provide the correct reading frame of the triplet code in a DNA sequence with inappropriate reading frame. Insulin A-chain and B-chain genes of the human type, have been synthesized with the appropriate adaptor molecules provided on each end. The adapted DNA genes have been joined to replicable cloning vehicles and the hybrid DNA transferred to a host cell. The transformed host cell has been shown to contain the desired insulin gene.

公开(公告)号：EP0068701A2

公开(公告)日：1983-01-05

申请号：EP82303071.3

申请日：1982-06-14

Applicant: Canadian Patents and Development Limited

Inventor： Narang, Saran A. ,  Wu, Ray J.

IPC: C12N15/00 ,  C12P21/02 ,  C07C103/52 ,  C07H21/04

CPC classification number: C12N15/66 ,  C07K14/62

Abstract: Human-like proinsulin gene analogs have been synthesized by a combination of chemical and enzymatic methods. A number of different human-like proinsulin gene analogs with altered C-chains have been designed and can be readily constructed as described. As a part of the strategy, an adaptor for trimming DNA has been used to recover the A-chain insulin gene with the desired sequence from a hybrid plasmid; a related adaptor for trimming DNA has been used to shorten the C-chain gene. The synthetic proinsulin gene has been joined to a replicable cloning vehicle and the hybrid DNA transferred to a host cell. The transformed host cells can be grown under selected conditions to yield the proinsulin analogs.

Abstract translation: 人类胰岛素原基因类似物已经通过化学和酶学方法的组合合成。 已经设计了许多具有改变的C链的人类类胰岛素原基因类似物，并且可以如所述容易地构建。 作为策略的一部分，已经使用用于修剪DNA的接头用于从杂交质粒中以期望的顺序恢复A链胰岛素基因; 已经使用用于修剪DNA的相关接头来缩短C链基因。 合成胰岛素原基因已经连接到可复制的克隆载体上，并将杂交DNA转移到宿主细胞。 转化的宿主细胞可以在选择的条件下生长以产生胰岛素原类似物。

公开(公告)号：EP0068701A3

公开(公告)日：1983-06-29

申请号：EP82303071

申请日：1982-06-14

Applicant: Canadian Patents and Development Limited

Inventor： Narang, Saran A. ,  Wu, Ray J.

IPC: C12N15/00 ,  C12P21/02 ,  C07C103/52 ,  C07H21/04

CPC classification number: C12N15/66 ,  C07K14/62

Abstract: Human-like proinsulin gene analogs have been synthesized by a combination of chemical and enzymatic methods. A number of different human-like proinsulin gene analogs with altered C-chains have been designed and can be readily constructed as described. As a part of the strategy, an adaptor for trimming DNA has been used to recover the A-chain insulin gene with the desired sequence from a hybrid plasmid; a related adaptor for trimming DNA has been used to shorten the C-chain gene. The synthetic proinsulin gene has been joined to a replicable cloning vehicle and the hybrid DNA transferred to a host cell. The transformed host cells can be grown under selected conditions to yield the proinsulin analogs.

公开(公告)号：EP0040466B1

公开(公告)日：1987-11-11

申请号：EP81301304.2

申请日：1981-03-26

Applicant: Canadian Patents and Development Limited

Inventor： Narang, Saran A. ,  Wu, Ray J.

IPC: C12N15/00 ,  C07H21/04

CPC classification number: C12N15/10 ,  C07K14/62 ,  C12N15/66 ,  C12N15/70

公开(公告)号：EP0040466A2

公开(公告)日：1981-11-25

申请号：EP81301304.2

申请日：1981-03-26

Applicant: Canadian Patents and Development Limited

Inventor： Narang, Saran A. ,  Wu, Ray J.

IPC: C12N15/00 ,  C07H21/04

CPC classification number: C12N15/10 ,  C07K14/62 ,  C12N15/66 ,  C12N15/70

Abstract: Adaptor molecules have been prepared to comprise either start or stop signals for protein synthesis, in addition to recognition sites for restriction endonucleases. Separate adaptors may be used in a symmetrical duplex form. The start adaptor may include nucleotide base inserts to provide the correct reading frame of the triplet code in a DNA sequence with inappropriate reading frame. Insulin A-chain and B-chain genes of the human type, have been synthesized with the appropriate adaptor molecules provided on each end. The adapted DNA genes have been joined to replicable cloning vehicles and the hybrid DNA transferred to a host cell. The transformed host cell has been shown to contain the desired insulin gene.

Abstract translation: 衔接分子已经准备包括开始或停止用于蛋白质合成的信号，除了对限制性内切酶识别位点。 单独的适配器可以以对称双链形式使用。起始适配器可包括核苷酸碱基插入在与不适当的阅读框的DNA序列，以提供三联体密码的正确阅读框。 人型的胰岛素A链和B链的基因，合成havebeen与设置在每个端部相应的适配器分子。 所述angepasst DNA基因已被连接到可复制的克隆载体，并转移到宿主细胞中的DNA杂交。 转化的宿主细胞已被证明含有所需的胰岛素基因。