公开(公告)号：DE69212998T2

公开(公告)日：1997-01-16

申请号：DE69212998

申请日：1992-03-13

Applicant: DADE CHEMISTRY SYSTEMS INC

Inventor： BUSSIAN RONALD WAYNE ,  RUGLASS JOHN ARTHUR

IPC: C12Q1/32

Abstract: The invention is drawn to a diagnostic assay for lactate dehydrogenase isoenzyme LD1 in a sample containing lactate dehydrogenase isoenzymes LD1-LD5. The disclosed assay involves the following steps: (A) adding a high molecular weight polyol, preferably polyethylene glycol having a molecular weight of 8000, to the sample to stabilize isoenzyme LD1 but not isoenzymes LD2-LD5; (B) adding an ionic amphiphile to the sample to selectively inactivate isoenzymes LD2-LD5; (C) adding sodium lactate and NAD to the sample; (D) determining LD1 by measuring a change in absorbance of the sample at 340 mm.

公开(公告)号：DE69212998D1

公开(公告)日：1996-09-26

申请号：DE69212998

申请日：1992-03-13

Applicant: DADE CHEMISTRY SYSTEMS INC

Inventor： BUSSIAN RONALD WAYNE ,  RUGLASS JOHN ARTHUR

IPC: C12Q1/32

Abstract: The invention is drawn to a diagnostic assay for lactate dehydrogenase isoenzyme LD1 in a sample containing lactate dehydrogenase isoenzymes LD1-LD5. The disclosed assay involves the following steps: (A) adding a high molecular weight polyol, preferably polyethylene glycol having a molecular weight of 8000, to the sample to stabilize isoenzyme LD1 but not isoenzymes LD2-LD5; (B) adding an ionic amphiphile to the sample to selectively inactivate isoenzymes LD2-LD5; (C) adding sodium lactate and NAD to the sample; (D) determining LD1 by measuring a change in absorbance of the sample at 340 mm.