公开(公告)号：DE69319693T2

公开(公告)日：1998-12-10

申请号：DE69319693

申请日：1993-09-09

Applicant: DADE CHEMISTRY SYSTEMS INC

Inventor： LOYD JOSEPH ,  SEARCH BARBARA A

IPC: C12N9/12 ,  C12N9/96 ,  C12Q1/50 ,  G01N33/573

Abstract: A formulation for stabilizing enzymatic activity and immunoreactivity of creatine kinase and creatine kinase isoenzymes comprising animal serum, creatine kinase and/or creatine kinase isoenzymes, an effective amount of a buffer having a pKa in the range of 6.2-7.6 to maintain pH of the formulation at 6.7+/-0.1, an effective amount of a non-thiol antioxidant to prevent oxidation of creatine kinase or creatine kinase isoenzyme thiol groups, an effective amount of a non-reducing polyol to stabilize immunoreactivity of creatine kinase or creatine kinase isoenzymes, and an effective amount of an antimicrobial to prevent microbial growth is provided.

公开(公告)号：DE69319693D1

公开(公告)日：1998-08-20

申请号：DE69319693

申请日：1993-09-09

Applicant: DADE CHEMISTRY SYSTEMS INC

Inventor： LOYD JOSEPH ,  SEARCH BARBARA A

IPC: C12N9/12 ,  C12N9/96 ,  C12Q1/50 ,  G01N33/573

Abstract: A formulation for stabilizing enzymatic activity and immunoreactivity of creatine kinase and creatine kinase isoenzymes comprising animal serum, creatine kinase and/or creatine kinase isoenzymes, an effective amount of a buffer having a pKa in the range of 6.2-7.6 to maintain pH of the formulation at 6.7+/-0.1, an effective amount of a non-thiol antioxidant to prevent oxidation of creatine kinase or creatine kinase isoenzyme thiol groups, an effective amount of a non-reducing polyol to stabilize immunoreactivity of creatine kinase or creatine kinase isoenzymes, and an effective amount of an antimicrobial to prevent microbial growth is provided.