公开(公告)号：DE69314462D1

公开(公告)日：1997-11-13

申请号：DE69314462

申请日：1993-08-24

Applicant: DADE MICROSCAN INC

Inventor： COAKLEY JOSEPH ,  GODSEY JAMES ,  SHERMAN DAVID

IPC: B01L3/00 ,  G01N1/00 ,  G01N35/00

Abstract: A sealable container for holding an analytical sample for processing is disclosed. The container includes a body with a dispensing port and an insert member with an outlet port slidably received in the body. The insert member and the body are movable relative to each other between a first position, in which the outlet port is substantially aligned with the dispensing port, and a second position, in which the outlet port is substantially out of alignment with the dispensing port. Sealing between the insert member and the body, which may take the form of O-rings, provide a substantially airtight, hermetic seal between a reaction chamber formed in the insert member and the body so that the reaction chamber can be sealed or isolated from the outside environment. The container is particularly adapted to contain the processes of nucleic acid amplification and to thereby reduce the problems of sample cross-contamination encountered in such processes. A method of processing an analytical sample using such a container is also disclosed.

公开(公告)号：DE69314462T2

公开(公告)日：1998-05-20

申请号：DE69314462

申请日：1993-08-24

Applicant: DADE MICROSCAN INC

Inventor： COAKLEY JOSEPH ,  GODSEY JAMES ,  SHERMAN DAVID

IPC: B01L3/00 ,  G01N1/00 ,  G01N35/00

Abstract: A sealable container for holding an analytical sample for processing is disclosed. The container includes a body with a dispensing port and an insert member with an outlet port slidably received in the body. The insert member and the body are movable relative to each other between a first position, in which the outlet port is substantially aligned with the dispensing port, and a second position, in which the outlet port is substantially out of alignment with the dispensing port. Sealing between the insert member and the body, which may take the form of O-rings, provide a substantially airtight, hermetic seal between a reaction chamber formed in the insert member and the body so that the reaction chamber can be sealed or isolated from the outside environment. The container is particularly adapted to contain the processes of nucleic acid amplification and to thereby reduce the problems of sample cross-contamination encountered in such processes. A method of processing an analytical sample using such a container is also disclosed.

公开(公告)号：DE69219989T2

公开(公告)日：1997-11-27

申请号：DE69219989

申请日：1992-04-29

Applicant: DADE MICROSCAN INC

Inventor： JOHNSTON JUDITH ,  FELLAND TRACY ,  BASCOMB SHOSHANA ,  GODSEY JAMES

IPC: C12Q1/18 ,  C12Q1/02 ,  C12Q1/20 ,  C12N1/38 ,  C12Q1/34

Abstract: In an assay for determination of beta-lactam antibiotic resistance in a target bacterial strain, the strain is grown in the presence of both a beta-lactamese inducing antibiotic and a beta-lactam indicator antibiotic [which kills or inhibits the growth of bacteria] unable to hydrolyze beta-lactam antibiotics. Growth, indicative of drug resistance in the target strain, is monitored by detecting a fluorophor released by the enzymatic cleavage of a metabolizable fluorogenic compound.

公开(公告)号：DE69219989D1

公开(公告)日：1997-07-03

申请号：DE69219989

申请日：1992-04-29

Applicant: DADE MICROSCAN INC

Inventor： JOHNSTON JUDITH ,  FELLAND TRACY ,  BASCOMB SHOSHANA ,  GODSEY JAMES

IPC: C12Q1/18 ,  C12Q1/02 ,  C12Q1/20 ,  C12N1/38 ,  C12Q1/34

Abstract: In an assay for determination of beta-lactam antibiotic resistance in a target bacterial strain, the strain is grown in the presence of both a beta-lactamese inducing antibiotic and a beta-lactam indicator antibiotic [which kills or inhibits the growth of bacteria] unable to hydrolyze beta-lactam antibiotics. Growth, indicative of drug resistance in the target strain, is monitored by detecting a fluorophor released by the enzymatic cleavage of a metabolizable fluorogenic compound.