公开(公告)号：WO2019003188A1

公开(公告)日：2019-01-03

申请号：PCT/IB2018/054823

申请日：2018-06-28

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： BABA, Takashi ,  LEBLANC, Yves

IPC: H01J49/42 ,  G01N33/68

Abstract: A system and method is described for characterizing glycopeptides which includes a first quadrupole mass filter, a multipole rod set of an ion guide, a lens electrode, an ExD device and a mass analyzer. The multipole rod set is adapted to receive a radial radio frequency (RF) trapping voltage and a radial dipole direct current (DC) voltage The lens electrode is adapted to receive an axial trapping alternating current (AC) voltage and a DC voltage. The ExD device performs electron capture dissociation or electron transfer dissociation, the ExD device being positioned so that an entrance of the ExD device is disposed on the other side of the lens electrode opposite the multipole rod set. The mass analyzer is positioned at an exit of the ExD device for receiving ions from the ExD device.

公开(公告)号：WO2017093898A1

公开(公告)日：2017-06-08

申请号：PCT/IB2016/057192

申请日：2016-11-29

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： CAMPBELL, John L. ,  LEBLANC, Yves ,  LIU, Chang

IPC: H01J49/00

CPC classification number: G01N33/58 ,  G01N27/624

Abstract: Methods and systems for separating and/or quantifying compounds, using differential mobility spectrometry (DMS) are provided herein. In accordance with various aspects of the applicants' teachings, the methods and systems can provide for the quantification of one or more compounds, for example, using isomeric labels that can be less costly to produce relative to conventional tags that incorporate stable-isotope labels. The present teachings can quantify the relative amount of a compound based on the effect of using an easily charged functional group as well as a functional group positioned at a resonant or non-resonant position through a DMS. In some aspects, methods and systems in accordance with various aspects of the present teachings provide for the detection and/or quantification of the analytes labeled with isomeric tags that can be differentiated via a DMS upstream of a first stage mass analyzer and/or prior to fragmentation of the labeled analyte, for example.

Abstract translation: 本文提供了使用差分迁移率谱（DMS）分离和/或定量化合物的方法和系统。 根据申请人的教导的各个方面，所述方法和系统可以提供一种或多种化合物的定量，例如使用异构标记，相对于掺入稳定同位素标记的常规标记，其生产成本可以较低。 本教导可以基于使用容易带电的官能团以及通过DMS定位在共振或非共振位置的官能团的效果来量化化合物的相对量。 在一些方面，根据本教导的各个方面的方法和系统提供了用异构体标签标记的分析物的检测和/或定量，所述异构体标签可以经由第一级质量分析器上游的DMS和/或在 例如，标记分析物的碎裂。

公开(公告)号：WO2017093896A1

公开(公告)日：2017-06-08

申请号：PCT/IB2016/057189

申请日：2016-11-29

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： ARNOLD, Don W. ,  CAMPBELL, John L. ,  COVEY, Thomas R. ,  LEBLANC, Yves ,  LIU, Chang

IPC: B03C5/00 ,  B03C1/14

CPC classification number: B03C1/30 ,  B03C1/0335 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  G01N35/0098

Abstract: Methods and apparatus for processing fluids are described. In various aspects, a fluid processing system may include a magnetic assembly that includes a plurality of magnetic structures configured to generate a magnetic field gradient within a fluid container. The magnetic structures may be formed as a plurality of electromagnets configured to be individually actuated by a controller. Each of the electromagnets may generate a magnetic field within the fluid container. The electromagnets may be differentially actuated to create a magnetic field gradient within the fluid container to agitate, mix, or otherwise influence magnetic particles disposed within the fluid container. Activation of the electromagnets of an electromagnetic structure may generate a magnetic field gradient that influences magnetic particles in an x-y direction. In addition, activation of the electromagnets of a plurality of electromagnetic structures may generate magnetic field gradients that influences magnetic particles in an x-y direction and z-direction.

Abstract translation: 描述了用于处理流体的方法和设备。 在各个方面，流体处理系统可以包括磁性组件，该磁性组件包括被配置为在流体容器内产生磁场梯度的多个磁性结构。 磁结构可以形成为配置成由控制器单独致动的多个电磁体。 每个电磁体可以在流体容器内产生磁场。 电磁体可以被差动致动以在流体容器内产生磁场梯度以搅动，混合或以其他方式影响设置在流体容器内的磁性粒子。 电磁结构的电磁体的激活可以产生影响磁性粒子在x-y方向上的磁场梯度。 另外，多个电磁结构的电磁体的激活可以产生影响磁性粒子在x-y方向和z方向上的磁场梯度。

公开(公告)号：WO2015170268A1

公开(公告)日：2015-11-12

申请号：PCT/IB2015/053313

申请日：2015-05-06

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： CAMPBELL, John Lawrence ,  CHOI, Kihwan ,  LEBLANC, Yves ,  LIU, Chang ,  WHEELER, Aaron

IPC: G01N35/10 ,  B01L3/00

CPC classification number: B01L3/502792 ,  B01L2300/0645 ,  B01L2300/0887 ,  B01L2400/0415 ,  B01L2400/0427 ,  G01N30/16 ,  G01N35/00871 ,  G01N2030/162

Abstract: Methods and systems for processing fluids utilizing a digital microfluidic device and transferring droplets from the digital microfluidic device to a downstream analyzer are described herein. Methods and systems in accordance with the present teachings can allow for the withdrawal of fluid from a digital microfluidic device, and can in some aspects enable the integration of a digital microfluidic device as a direct, in-line sample processing platform from which a droplet can be transferred to a downstream analyzer.

Abstract translation: 本文描述了利用数字微流体装置处理流体并将液滴从数字微流体装置转移到下游分析器的方法和系统。 根据本教导的方法和系统可以允许从数字微流体装置中取出流体，并且在某些方面，可以将数字微流体装置整合为直接的在线样品处理平台，液滴可从该样品处理平台 转移到下游分析仪。

公开(公告)号：WO2013171574A1

公开(公告)日：2013-11-21

申请号：PCT/IB2013/000976

申请日：2013-05-17

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： CAMPBELL, John, L. ,  COVEY, Thomas ,  LEBLANC, Yves ,  SCHNEIDER, Bradley

IPC: H01J49/04 ,  H01J49/10

CPC classification number: H01J49/165 ,  G01N27/622 ,  H01J49/0081 ,  H01J49/0431

Abstract: An electrospray ion source method and system is provided for detecting emitter failure comprising a liquid chromatography column suitable for chromatographic separation of a sample. The column can have an inlet for receiving the sample; and an outlet for ejecting the sample. A make-up flow channel is provided for introducing make-up flow of liquid to the sample post-column, wherein the make-up flow normalizes the spray current. An electrospray ionization source is provided having one or more electrospray ionization emitter nozzles for receiving the make-up flow containing sample. A power supply can provide a voltage to the one or more emitter nozzles, and a measurement device can measure and monitor the spray current.

Abstract translation: 提供了一种用于检测发射器故障的电喷雾离子源方法和系统，其包括适于样品色谱分离的液相色谱柱。 该柱可以具有用于接收样品的入口; 以及用于喷射样品的出口。 提供了补充流动通道，用于将液体的补充流引入样品后柱，其中补充流使标准化喷雾电流。 提供了具有一个或多个电喷雾电离发射器喷嘴的电喷雾离子源，用于接收含有补充流的样品。 电源可以向一个或多个发射器喷嘴提供电压，并且测量装置可以测量和监测喷射电流。

公开(公告)号：WO2011031665A2

公开(公告)日：2011-03-17

申请号：PCT/US2010/047970

申请日：2010-09-07

Applicant: PURDUE RESEARCH FOUNDATION ,  DH TECHNOLOGIES DEVELOPMENT PTE. LTD. ,  HASSELL, Kerry, Marie ,  MCLUCKEY, Scott, A. ,  LEBLANC, Yves

Inventor： HASSELL, Kerry, Marie ,  MCLUCKEY, Scott, A. ,  LEBLANC, Yves

IPC: G01N30/72 ,  G01N27/62 ,  G01N30/12

CPC classification number: H01J49/0072 ,  H01J49/004 ,  H01J49/165 ,  Y10T436/24

Abstract: An apparatus and method for a sample using a mass spectrometer is described, including, generating ions of a first polarity from an analyte using electrospray ionization; generating ions of a second polarity from a reagent; injecting the ions of the first polarity and ions of the second polarity in sequence into a chamber of the mass spectrometer such that the ions of the first polarity and the ions of the second polarity interact in the chamber to form analyte ions having the second polarity; and, analyzing the mass spectrum of the analyte ions of the second polarity. A reagent such as a polyamidomine is selected to preferentially yield analyte ions of the second polarity having a desired mass-to-charge ratio.

Abstract translation: 描述了使用质谱仪的样品的装置和方法，包括使用电喷雾离子化从分析物产生具有第一极性的离子; 从试剂产生第二极性的离子; 将第一极性的离子和第二极性的离子依次注入质谱仪的室中，使得第一极性的离子和第二极性的离子在室中相互作用以形成具有第二极性的分析物离子; 并分析第二极性的分析物离子的质谱。 选择诸如聚酰胺胺的试剂优先产生具有所需质荷比的具有第二极性的分析物离子。

公开(公告)号：WO2022264091A1

公开(公告)日：2022-12-22

申请号：PCT/IB2022/055615

申请日：2022-06-16

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： SCHNEIDER, Bradley B. ,  BEDFORD, Leigh ,  LEBLANC, Yves ,  DUCHOSLAV, Eva

IPC: G01N27/624

Abstract: An automated method of operating a mass spectrometer (MS) comprising a differential mobility spectrometer (DMS) includes: introducing a first compound to the DMS; calculating a first alpha function for the first compound; introducing a second compound to the DMS; calculating a second alpha function for the second compound; and determining operation parameters of the DMS to achieve sufficient separation of the first compound and the second compound, based on the first alpha function and the second alpha function.

公开(公告)号：WO2016030860A1

公开(公告)日：2016-03-03

申请号：PCT/IB2015/056534

申请日：2015-08-28

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： CAMPBELL, John ,  LEBLANC, Yves ,  LIU, Chang

IPC: G01N27/62 ,  H01J49/00 ,  H01J49/04 ,  H01J49/26

CPC classification number: G01N27/624 ,  G01N33/6803 ,  H01J49/04 ,  H01J49/10

Abstract: Methods and systems for separating and/or quantifying peptides using differential mobility spectrometry (DMS) are provided herein. In accordance with various aspects of the applicant's teachings, the methods and systems can provide for the separation of one or more peptides, for example, peptides that may be difficult to separate with conventional techniques, such as mass spectrometry (MS), by complexing the peptides with a metal cation (e.g., Ca2+) prior to DMS. In some aspects, the present teachings can prevent proton stripping from ionized peptides that can occur in DMS to prevent unintended and/or undesirable alterations to the peptide's charge state distribution.

Abstract translation: 本文提供了使用差分迁移谱（DMS）分离和/或定量肽的方法和系统。 根据申请人的教导的各个方面，所述方法和系统可以提供一种或多种肽的分离，例如可能难以与常规技术（例如质谱（MS））分离的肽，通过使 在DMS之前具有金属阳离子（例如，Ca 2+）的肽。 在一些方面，本发明教导可以防止可能发生在DMS中的离子化肽的质子剥离，以防止肽的电荷状态分布的非预期和/或不期望的改变。

公开(公告)号：WO2013098603A1

公开(公告)日：2013-07-04

申请号：PCT/IB2012/002530

申请日：2012-11-28

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： CAMPBELL, John, L. ,  LEBLANC, Yves ,  LOBODA, Alexandre ,  CHERNUSEVICH, Igor V.

IPC: H01J49/26

CPC classification number: G06F19/703 ,  H01J49/0031 ,  H01J49/004

Abstract: In a first location of a mass spectrometer, a plurality of ionized molecules of an ion source are selected that have mass-to-charge ratios within a mass-to-charge ratio window width. The plurality of selected ionized molecules are transmitted from a first to a second location. Reagent ions are transmitted to the second location to reduce a charge state of one or more of the plurality of selected ionized molecules. A mass analyzer is used to analyze the plurality of reduced ionized molecules and produce a mass spectrum. A compound is identified from a peak of the spectrum that has a mass-to-charge ratio less than or equal to the highest mass-to-charge ratio in the window width if the noise is multiply charged and greater than the highest mass-to-charge ratio in the window width if the noise is singly charged.

Abstract translation: 在质谱仪的第一位置，选择离子源的多个离子化分子，其具有在质荷比窗口宽度之内的质荷比。 多个选择的电离分子从第一位置传递到第二位置。 试剂离子被传递到第二位置以减少多个选定的电离分子中的一个或多个的电荷状态。 质量分析器用于分析多个还原离子化分子并产生质谱。 如果噪声是多次充电并且大于最高质量 - 到达的质量 - 荷电比，如果光谱的峰值具有小于或等于窗口宽度中的最高质荷比的化合物， 如果噪声被单独充电，则窗口宽度的充电比例。

公开(公告)号：WO2018055483A1

公开(公告)日：2018-03-29

申请号：PCT/IB2017/055541

申请日：2017-09-13

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： COVEY, Thomas R. ,  LEBLANC, Yves ,  SCHNEIDER, Bradley A.

IPC: H01J49/10 ,  H01J49/26

Abstract: Because most ion optics of mass spectrometry systems are subject to ion deposition and may exhibit significantly different behavior following substantial contamination (e.g., loss of sensitivity), fouled surfaces must be regularly cleaned to maintain sensitivity. While the surfaces of front-end components (e.g., curtain plates, orifice plates, Qjet, Q0, IQ0) may be relatively easy to clean, the fouling of components contained within the downstream high-vacuum chambers (e.g., Q1, IQ1) can incur substantial delays and expense as the high-vacuum chambers must be vented and substantially disassembled prior to cleaning. Methods and systems for controlling contamination of components of mass spectrometer systems are provided herein. By reducing the transmission of contaminating ions during non-data acquisition periods, the present teachings can increase throughput, improve robustness, and/or decrease the downtime typically required to vent/disassemble/clean the fouled components.

Abstract translation: 由于质谱系统的大多数离子光学系统都受到离子沉积的影响，并且在大量污染（例如，灵敏度损失）后可能表现出显着不同的行为，必须定期清洁污垢表面以保持灵敏度。 尽管前端部件（例如，帘板，孔板，Qjet，Q0，IQ0）的表面可能相对容易清洁，但包含在下游高真空室（例如Q1，IQ1）内的部件的污垢可以 由于高真空室在清洁之前必须排气并且基本上拆卸，所以会产生实质性的延迟和费用。 本文提供了用于控制质谱仪系统的部件的污染的方法和系统。 通过在非数据采集周期期间减少污染离子的传输，本教导可以增加吞吐量，改善鲁棒性和/或减少通常/拆卸/清洁污染组件所需的停机时间。