公开(公告)号：AU2021377284A9

公开(公告)日：2024-10-17

申请号：AU2021377284

申请日：2021-11-15

Applicant: DONALDSON COMPANY INC

Inventor： ZHOU JINXIANG ,  TEMPLES GRAHAM

IPC: C07H21/00 ,  C07K1/22 ,  C12Q1/6806 ,  G01N33/543

Abstract: Disclosed are methods for purifying polynucleotides from a feed solution using oligonucleotide affinity columns at flowrates between 0.5 CV/min to 1000 CV/min. The methods can include several steps including loading a mixture containing the target polynucleotide onto a chromatography media, e.g., a column, that carries a macroporous support with an oligonucleotide affinity ligand bonded to a surface. The affinity ligand can hybridize the targeted polynucleotide and allow for separation and purification of the target.