公开(公告)号：US20220098610A1

公开(公告)日：2022-03-31

申请号：US17403476

申请日：2021-08-16

Applicant: DOW AGROSCIENCES LLC

Inventor： Terry WRIGHT ,  Justin LIRA ,  Donald MERLO ,  Nicole ARNOLD

IPC: C12N15/82 ,  C12N9/02

Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.

公开(公告)号：US20170022515A1

公开(公告)日：2017-01-26

申请号：US15288406

申请日：2016-10-07

Applicant: Dow AgroSciences LLC

Inventor： Terry WRIGHT ,  Justin LIRA ,  Donald MERLO ,  Nicole ARNOLD

IPC: C12N15/82 ,  A01N57/20 ,  A01N43/60 ,  C12N9/02 ,  A01N37/38

CPC classification number: C12N15/8274 ,  C12N9/0069 ,  C12N15/8209

Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.

Abstract translation: 本发明提供了不仅对2,4-D和其它苯氧基生长素除草剂具有抗性的新型植物，而且还涉及芳氧基苯氧基丙酸酯除草剂。 迄今为止，没有任何期望或建议，通过引入单一基因可以产生具有这两种有利性质的植物。 本发明还包括单独产生本发明的一种或多种酶或与另一种除草剂抗性基因“优选地基于草甘膦抗性”基因“堆叠”的植物，以便提供更广泛和更强大的杂草控制，增加的治疗灵活性，和 改善除草剂抗性管理方案。 更具体地，根据本发明使用的优选的酶和基因在本文中称为AAD（芳氧基链烷酸酯加氧酶）基因和蛋白质。 据报道，没有α-酮戊二酸依赖性双加氧酶具有降解不同化学类别和作用模式的除草剂的能力。 这种高度新颖的发现是显着的除草剂耐受性作物性状机会以及可选择标记技术的发展的基础。 本发明还包括控制杂草的相关方法。 本发明能够以新的方式使用除草剂的新型组合。 此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性（或天然更耐受）的杂草的形成和控制的新方法。

公开(公告)号：US20210147867A1

公开(公告)日：2021-05-20

申请号：US17143824

申请日：2021-01-07

Applicant: DOW AGROSCIENCES LLC

Inventor： Terry WRIGHT ,  Justin LIRA ,  Donald MERLO ,  Nicole ARNOLD

IPC: C12N15/82 ,  C12N9/02

Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.

公开(公告)号：US20210403934A1

公开(公告)日：2021-12-30

申请号：US17386891

申请日：2021-07-28

Applicant: DOW AGROSCIENCES LLC

Inventor： Yunxing Cory CUI ,  Jill BRYAN ,  Donald MAUM ,  Greg GILLES ,  Terry WRIGHT ,  Jennifer HAMILTON ,  Nicole ARNOLD ,  Nathan VanOpdorp ,  Tina KAISER ,  Ning ZHOU

IPC: C12N15/82 ,  C12Q1/6895 ,  C12N9/02

Abstract: This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel AAD-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (or corn grain, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the corn genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided.

公开(公告)号：US20190233904A1

公开(公告)日：2019-08-01

申请号：US16279055

申请日：2019-02-19

Applicant: DOW AGROSCIENCES LLC

Inventor： Yunxing Cory CUI ,  Jill BRYAN ,  Donald MAUM ,  Greg GILLES ,  Terry WRIGHT ,  Jennifer HAMILTON ,  Nicole ARNOLD ,  Nathan VanOpdorp ,  Tina KAISER ,  Ning ZHOU

IPC: C12Q1/6895 ,  C12N15/82 ,  A01H5/10

CPC classification number: C12Q1/6895 ,  A01H5/10 ,  C12N9/0069 ,  C12N9/0071 ,  C12N15/8274 ,  C12Q2600/16 ,  C12Y113/00

Abstract: This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel aad-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (or corn grain, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the corn genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided.

公开(公告)号：US20210254090A1

公开(公告)日：2021-08-19

申请号：US17175966

申请日：2021-02-15

Applicant: DOW AGROSCIENCES LLC

Inventor： Terry WRIGHT ,  Justin LIRA ,  Donald MERLO ,  Nicole ARNOLD

IPC: C12N15/82 ,  C12N9/02

Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.