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    ACETYL-COA PRODUCING ENZYMES IN YEAST
    1.
    发明申请
    ACETYL-COA PRODUCING ENZYMES IN YEAST 审中-公开
    乙酰乙酸生产酶在酵母中的应用

    公开(公告)号：WO2009013159A2

    公开(公告)日：2009-01-29

    申请号：PCT/EP2008059119

    申请日：2008-07-11

    Applicant: DSM IP ASSETS BV ,  MUELLER ULRIKE MARIA ,  WU LIANG ,  RAAMSDONK LOURINA MADELEINE ,  WINKLER AARON ADRIAAN

    Inventor： MUELLER ULRIKE MARIA ,  WU LIANG ,  RAAMSDONK LOURINA MADELEINE ,  WINKLER AARON ADRIAAN

    IPC: C12N15/81 ,  C12P7/16

    CPC classification number: C12Q1/32 ,  C07K14/33 ,  C12N9/001 ,  C12P7/16 ,  Y02E50/10

    Abstract: The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grow on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) said yeast cell when growth of said cell is observed. The invention further relates to a method of producing a fermentation production such as butanol.

    Abstract translation: 本发明涉及鉴定具有酶促活性的异源多肽的方法，所述酶促活性用于将丙酮酸，乙醛或乙酸转化为酵母细胞（的胞质溶胶）中的乙酰-CoA，所述方法包括：a）提供突变的酵母细胞，所述突变的酵母细胞包含至少 选自编码丙酮酸脱羧酶（PDC），乙醛脱氢酶（ALD）和乙酰-CoA合成酶（ACS）的基因的（PDH）旁路的一个基因; b）用表达载体转化所述突变的酵母细胞，所述表达载体包含编码具有用于将丙酮酸，乙醛或乙酸转化成乙酰-CoA的潜在酶活性的候选多肽的异源核苷酸序列; c）测试所述重组突变的酵母细胞在含有葡萄糖作为唯一碳源的基本培养基上生长的能力，和d）将所述候选多肽鉴定为具有用于将丙酮酸，乙醛或乙酸转化为乙酰辅酶A的酶活性的异源多肽 当观察到所述细胞的生长时，所述酵母细胞的胞质溶胶）。 本发明进一步涉及生产发酵生产如丁醇的方法。

    PROCESS FOR THE PREPARATION OF BUTANOL AND ETHANOL
    2.
    发明申请
    PROCESS FOR THE PREPARATION OF BUTANOL AND ETHANOL 审中-公开
    丁醇和乙醇的制备方法

    公开(公告)号：WO2009013160A2

    公开(公告)日：2009-01-29

    申请号：PCT/EP2008059120

    申请日：2008-07-11

    Applicant: DSM IP ASSETS BV ,  DIJK EINTE-KARST ,  RAAMSDONK LOURINA MADELEINE

    Inventor： DIJK EINTE-KARST ,  RAAMSDONK LOURINA MADELEINE

    IPC: C12P7/16 ,  C12N9/02 ,  C12N15/81

    CPC classification number: C12P7/16 ,  B01D3/002 ,  C12P7/06 ,  C12R1/865 ,  Y02E50/10 ,  Y02E50/17

    Abstract: The present invention relates to a eukaryotic cell capable of producing butanol and ethanol at a ratio butanol: ethanol of between 1:2 to 1:100. The present invention further relates to a process for the preparation of butanol and ethanol comprising fermenting a eukaryotic cell in a suitable fermentation broth, wherein butanol and ethanol are produced at a ratio butanol: hethanol of between 1:2 to 1:100 and a process for the recovery of butanol and ethanol from an aqueous solution comprising butanol and ethanol wherein the ratio butanol: ethanol is between 1:2 to 1:100 comprising, separating an ethanol/butanol/water mixture from the aqueous solution; separating an ethanol/water mixture from the ethanol/butanol/water mixture; separating a butanol/water mixture from the ethanol/butanol/water mixture; and recovering of butanol and ethanol.

    Abstract translation: 本发明涉及能够以1:2至1:100的丁醇/乙醇比例生产丁醇和乙醇的真核细胞。 本发明还涉及一种制备丁醇和乙醇的方法，包括在合适的发酵液中发酵真核细胞，其中丁醇和乙醇以1:2至1:100的丁醇：乙醇的比例生产， 用于从包含丁醇和乙醇的水溶液中回收丁醇和乙醇，其中丁醇：乙醇的比例为1:2至1:100，包括从水溶液中分离出乙醇/丁醇/水混合物; 从乙醇/丁醇/水混合物中分离乙醇/水混合物; 从乙醇/丁醇/水混合物中分离出丁醇/水混合物; 并回收丁醇和乙醇。

    9.
    发明专利
    未知

    公开(公告)号：BRPI0813710A2

    公开(公告)日：2014-10-07

    申请号：BRPI0813710

    申请日：2008-07-11

    Applicant: DSM IP ASSETS BV

    Inventor： MUELLER ULRIKE MARIA ,  WU LIANG ,  RAAMSDONK LOURINA MADELEINE ,  WINKLER AARON ADRIAAN

    IPC: C12N15/81 ,  C12P7/16

    Abstract: The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) of a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grown on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) said yeast cell when growth of said cell is observed. The invention further relates to a method of producing a fermentation production such as butanol.

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