公开(公告)号：WO2013144898A3

公开(公告)日：2014-01-23

申请号：PCT/IB2013052493

申请日：2013-03-28

Applicant: ECOLE POLYTECH

Inventor： PAPADOPOULOS IOANNIS ,  FARAHI SALMA ,  MOSER CHRISTOPHE ,  PSALTIS DEMETRI

IPC: F21V8/00 ,  G02B6/028 ,  G03H1/00

CPC classification number: H04N1/40 ,  G01J3/0218 ,  G01J3/44 ,  G02B6/0288 ,  G02B23/26 ,  G02B26/06 ,  G02B27/58 ,  G02F1/011 ,  G02F1/0136 ,  G02F1/313 ,  G03H1/0005 ,  G03H1/2286 ,  G03H1/2294 ,  G03H2001/0434 ,  G03H2001/0447 ,  H04B10/2581

Abstract: A multimode waveguide illuminator and imager relies on a wave front shaping system that acts to compensate for modal scrambling and light dispersion by the multimode waveguide. A first step consists of calibrating the multimode wave¬ guide and a second step consists in projecting a specific pattern on the wave¬ guide proximal end in order to produce the desire light pattern at its distal end. The illumination pattern can be scanned or changed dynamically only by chang¬ ing the phase pattern projected at the proximal end of the waveguide. The third and last step consists in collecting the optical information, generated by the sample, through the same waveguide in order to form an image. Known free space microscopy technique can be adapted to endoscopy with multimode waveguide, such as, but not limited to, fluorescence imaging or Raman spectros¬ copy or imaging, 3D linear scattering imaging or two-photon imaging. Super- resolution, i.e., resolution below the diffraction limit, is achieved for example but not limited to, using the STimulated Emission Depletion microscopy (STED) technique or the Structured Illumination Microscopy (SIM) technique or a stochastic illumination based method (PALM, STORM) in combination with the multimode waveguide imaging method.

Abstract translation: 多模波导照明器和成像器依赖于波前整形系统，其用于补偿多模波导的模式加扰和光色散。 第一步包括校准多模波导，第二步包括将特定图案投影在波导近端上，以在其远端产生期望的光图案。 可以通过改变在波导的近端处投影的相位图案来动态扫描或改变照明图案。 第三和最后一步是通过相同的波导收集由样品产生的光学信息，以形成图像。 已知的自由空间显微技术可以适用于多模波导的内窥镜检查，例如但不限于荧光成像或拉曼光谱复制或成像，3D线性散射成像或双光子成像。 超分辨率，即低于衍射极限的分辨率，例如但不限于使用受激发射耗尽显微镜（STED）技术或结构照明显微镜（SIM）技术或基于随机照明的方法（PALM，STORM ）结合多模波导成像方法。