公开(公告)号：US20240191265A1

公开(公告)日：2024-06-13

申请号：US18509425

申请日：2023-11-15

Applicant: ExxonMobil Technology and Engineering Company

Inventor： Xiaozhou Zhang ,  Glen E. Alliger ,  Yuchen Liu

IPC: C12P7/62

CPC classification number: C12P7/62

Abstract: Methods of β-lactone production, including the integrated bioconversion of β-lactone production and methods related thereto. Methods include reacting a feedstock and a recombinant microorganism harboring one or more genes encoding enzymes involved in the metabolic pathway of a 3-hydroxycarboxylic acid, thereby producing a 3-hydroxycarboxylic acid having C1-C10 alkyl groups. Thereafter, reacting the 3-hydroxycarboxilic acid with a bioengineered natural β-lactone synthetase, the bioengineered β-lactone synthetase engineered to comprise a substrate binding pocket capable of accepting the 3-hydroxycarboxylic acid having C1-C10 alkyl groups, thereby producing a β-lactone having C1-C10 alkyl groups.