公开(公告)号：US20160123970A1

公开(公告)日：2016-05-05

申请号：US14894287

申请日：2014-05-22

Applicant: FLUIDIGM CANADA INC.

Inventor： Dmitry R. BANDURA ,  Vladimir I. BARANOV ,  Alexander V. LOBODA ,  Olga ORNATSKY ,  Scott D. TANNER

IPC: G01N33/543 ,  G01N15/10 ,  G01N33/532 ,  G01N27/62

CPC classification number: G01N33/54366 ,  G01N15/1031 ,  G01N15/1404 ,  G01N15/1459 ,  G01N27/622 ,  G01N27/624 ,  G01N33/483 ,  G01N33/532 ,  G01N33/54313 ,  G01N2015/1006 ,  G01N2458/15

Abstract: The invention relates to methods and devices for molecular cytometry. The molecular cytometer is for use in the analysis of molecular tag labeled particles. In the molecular cytometer, volatile molecular tags attached to the particles, such as cells, are released as groups of molecular tags and ionized by the method of soft ionization to produce corresponding groups of molecular ions. The molecular cytometer has two detectors, one to detect the presence of the groups of molecular ions and the other to detect mobility separated molecular ions. The mobility separated molecular ions are synchronized to correspond with the groups of molecular ions.

Abstract translation: 本发明涉及用于分子细胞计数的方法和装置。 分子光谱仪用于分子标签标记颗粒的分析。 在分子细胞仪中，附着于粒子（如细胞）的挥发性分子标记作为分子标签的基团释放，并通过软电离方法离子化以产生相应的分子离子组。 分子细胞仪有两个检测器，一个用于检测分子离子组的存在，另一个用于检测迁移率分离的分子离子。 迁移率分离的分子离子被同步以对应于分子离子的组。

公开(公告)号：US20190180996A1

公开(公告)日：2019-06-13

申请号：US16322418

申请日：2017-08-02

Applicant: Fluidigm Canada Inc.

Inventor： Alexander V. LOBODA

IPC: H01J49/04 ,  H01J49/10 ,  H01J49/00

Abstract: The invention relates to methods and devices for analysis of samples using laser ablation imaging mass cytometry and mass spectrometry. The invention provides methods and devices in which individual ablation plumes are distinctively captured and rapidly transferred to the ionization system, followed by analysis by mass spectrometry. A transfer conduit can be used to convey ablation plumes to an ionization system. The transfer conduit can include an asymmetric cone. The transfer conduit can be tapered. A flow sacrificing system can be adapted to divert a part of the sheath flow out a sacrificial outlet while the core of the sheath flow containing ablation plumes enters the ionization system.