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    MASS CYTOMETRY REAGENTS AND METHODS FOR SIGNAL AMPLIFICATION
    1.
    发明申请
    MASS CYTOMETRY REAGENTS AND METHODS FOR SIGNAL AMPLIFICATION 审中-公开

    公开(公告)号：WO2021127435A1

    公开(公告)日：2021-06-24

    申请号：PCT/US2020/066012

    申请日：2020-12-18

    Applicant: FLUIDIGM CANADA INC.

    Inventor： ALLO, Bedilu ,  LOU, Xudong ,  LIU, Peng ,  MAJONIS, Daniel ,  MAVROPOULOS, Anastasia ,  GHEIRATMAND, Ladan

    IPC: C08F20/10 ,  C08F20/56 ,  C08F220/36 ,  C08F220/54 ,  C08F220/56

    Abstract: Described herein are reagents and methods for improving signal in imaging mass cytometry. Aspects include mass tags with a large number of labeling atoms, chemical modifications to mass tags and additional reagents to reduce background and/or maintain target binding of mass tagged specific binding partners (SBPs), and schemes for associating a plurality of mass tags with a single SBP. As such, embodiments include any combination of one or more reagents and their use. The reagents, kits and methods herein may be used for mass cytometry, including imaging mass cytometry. In some aspects, reagents, kits or methods may be used for delivery of a large number of radioisotopes to a target analyte, for example for therapeutic use or radiometric detection. In certain aspects, only non-radioactive isotopes may be used for mass cytometry.

    METHOD FOR PERFORMING MULTIPLEX ANALYSIS OF TWO OR MORE ANALYTES
    2.
    发明公开
    METHOD FOR PERFORMING MULTIPLEX ANALYSIS OF TWO OR MORE ANALYTES 审中-公开
    用于执行两种或更多种分析的多重分析的方法

    公开(公告)号：EP3260863A1

    公开(公告)日：2017-12-27

    申请号：EP17176041.6

    申请日：2007-05-28

    Applicant: Fluidigm Canada Inc.

    Inventor： WINNIK, Mitchell, A. ,  NITZ, Mark ,  BARANOV, Vladimir ,  LOU, Xudong

    IPC: G01N33/53 ,  G01N33/58 ,  C08F8/42 ,  C08F220/36

    CPC classification number: C08F8/32 ,  C08F8/14 ,  C08F8/42 ,  C08F220/36 ,  C08F220/56 ,  G01N33/532 ,  G01N33/57426 ,  G01N33/58 ,  Y10T436/24

    Abstract: The present invention relates to a method of performing multiplex analysis of two or more analytes, comprising: incubating two or more differential element tagged affinity reagents with two or more analytes, wherein each of the differential element tagged affinity reagents includes: an affinity reagent capable of binding to an analyte; and an element tag comprising a polymer linked to the affinity reagent, wherein the polymer comprises at least one metal-binding pendant group that comprises at least one metal atom; separating unbound affinity reagents from bound affinity reagents; and analyzing the differential tags bound to the two or more analytes by elemental analysis, wherein the element tag of one of the two or more differential element tagged affinity reagents is distinguishable from the element tag of another of the two or more differential element tagged affinity reagents through elemental analysis.

    Abstract translation: 包括：将两种或更多种差异元件标记的亲和试剂与两种或更多种分析物一起温育，其中每种差异元件标记的亲和试剂包括：亲和试剂，其能够 与分析物结合; 和包含与所述亲和试剂连接的聚合物的元件标签，其中所述聚合物包含至少一个包含至少一个金属原子的金属结合侧基; 从未结合的亲和试剂分离未结合的亲和试剂; 以及通过元素分析分析与两种或更多种分析物结合的差异标签，其中两种或更多种差异元件标记的亲和试剂之一的元件标签与两种或更多种差别元件标记的亲和试剂中的另一种的元件标签是可区分的 通过元素分析。

    POLYMER BACKBONE ELEMENT TAGS
    3.
    发明公开
    POLYMER BACKBONE ELEMENT TAGS 有权
    POLYMERRÜCKGRATELEMENTETIKETTEN

    公开(公告)号：EP2960263A1

    公开(公告)日：2015-12-30

    申请号：EP15177058.3

    申请日：2007-05-28

    Applicant: Fluidigm Canada Inc.

    Inventor： WINNIK, Mitchell, A. ,  NITZ, Mark ,  BARANOV, Vladimir ,  LOU, Xudong

    IPC: C08F220/36 ,  C08F8/14 ,  C08F8/32 ,  C08F8/42 ,  G01N33/532 ,  G01N33/58

    CPC classification number: C08F8/32 ,  C08F8/14 ,  C08F8/42 ,  C08F220/36 ,  C08F220/56 ,  G01N33/532 ,  G01N33/57426 ,  G01N33/58 ,  Y10T436/24

    Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats : bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).

    Abstract translation: 提供基于新型金属 - 聚合物共轭物的元件标签用于元素分析，包括ICP-MS。 聚合物骨架被官能化以不可逆地结合在使用者使用之前选择的金属。 该聚合物进一步官能化以连接允许附着于抗体或其它亲和试剂的接头。 聚合物形式允许附加许多拷贝的给定同位素，其线性地提高灵敏度。 金属 - 聚合物缀合物标签使得能够以两种形式进行多重测定：体积测定，其中样品中的平均生物标志物分布是诊断的，以及单细胞格式以区分复杂样品中的罕见（例如患病）细胞（例如， 血液）。

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