公开(公告)号：US20190077890A1

公开(公告)日：2019-03-14

申请号：US16047927

申请日：2018-07-27

Applicant: FLUIDIGM CANADA INC.

Inventor： MITCHELL A. WINNIK ,  MARK NITZ ,  VLADIMIR BARANOV ,  XUDONG LOU

IPC: C08F8/32 ,  G01N33/58 ,  G01N33/532 ,  C08F220/36 ,  G01N33/574 ,  C08F8/14 ,  C08F8/42 ,  C08F220/56

Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).

公开(公告)号：US20160025731A1

公开(公告)日：2016-01-28

申请号：US14875553

申请日：2015-10-05

Applicant: FLUIDIGM CANADA INC.

Inventor： MITCHELL A. WINNIK ,  MARK NITZ ,  VLADIMIR BARANOV ,  XUDONG LOU

IPC: G01N33/574 ,  C08F220/56

CPC classification number: C08F8/32 ,  C08F8/14 ,  C08F8/42 ,  C08F220/36 ,  C08F220/56 ,  G01N33/532 ,  G01N33/57426 ,  G01N33/58 ,  Y10T436/24

Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).