METHOD OF CORRECTING FOR AN AMPLITUDE CHANGE IN A SPECTROMETER

    公开(公告)号:WO2021038316A1

    公开(公告)日:2021-03-04

    申请号:PCT/IB2020/055099

    申请日:2020-05-29

    Abstract: A method of correcting for an amplitude change in a spectrometric instrument (300) output due to changes in an optical path length through the sample holder (600), the method comprising: exposing a sample in a sample holder (600) to electromagnetic radiation at a plurality of wavenumbers; detecting electromagnetic absorption intensities in the sample at the plurality of wavenumbers; providing to a computer device (510) the detected absorption intensities indexed against wavenumber as spectral data; and applying in the computer device (510) a mathematical transform (lcorr) to the spectral data to correct for an amplitude change in the spectrometric instrument's (300) output and calculated by determining a difference (Δ(SBz)') between absorbance values at two different wavenumber ranges in a first derivative of spectral data (SBz1) from a zero material sample; and calculating the mathematical transform (lcorr) as a function inversely dependent on the determined difference (Δ(SBz)')

    METHOD OF DETERMINING AN OPTICAL PATHLENGTH THROUGH A CUVETTE

    公开(公告)号:WO2021144630A1

    公开(公告)日:2021-07-22

    申请号:PCT/IB2020/059690

    申请日:2020-10-15

    Abstract: A method for determining an optical pathlength (L) of a cuvette of a spectrophotometric apparatus comprising: obtaining (720) a single beam spectrum (SBZ) of a liquid zero-material at least in a first energy region in which the liquid zero-material absorbs; obtaining (740) a single beam spectrum (SB2) of a second liquid at least in the first energy region, the second liquid having a composition excluding the liquid zero-material and having no absorption in the first energy region; determining (760) a dual beam spectrum (DBZ) of the liquid zero-material relative to the second liquid at least in the first energy region from the two obtained single beam spectra (SBZ; SB2); and calculating (780) an optical pathlength (L) through the cuvette dependant on spectral information obtained from the first energy region of the determined dual beam spectrum (DBZ).

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