公开(公告)号：US20180196009A1

公开(公告)日：2018-07-12

申请号：US15741673

申请日：2016-07-01

Applicant: Foundation for Research and Technology Hellas

Inventor： George PAPADAKIS ,  Elektra GIZELI

IPC: G01N29/02 ,  G01N29/032 ,  G01N29/036 ,  C12Q1/6825 ,  G01N33/58 ,  G01N33/543

CPC classification number: G01N29/022 ,  C12Q1/6825 ,  C12Q2565/634 ,  G01N29/032 ,  G01N29/036 ,  G01N33/54373 ,  G01N33/586 ,  G01N2291/0255 ,  G01N2291/0256 ,  G01N2291/0257 ,  G01N2291/0426 ,  C12Q2525/197

Abstract: A sensitive assay for an analyte employing an acoustic wave sensor. A label which has a higher dissipative capacity than the analyte is adhered to the sensing surface of an acoustic wave sensor through the analyte such that the body of the label is spaced apart from and anchored to the surface of the acoustic wave sensor by a distance of 15 to 250 nm. The change in the energy losses of the acoustic wave when the label binds to the sensing surface is used to measure the presence or amount of the label. A substantial improvement in the detection limit of the label is obtained. The analyte may for example be a nucleic acid and the label may for example comprise liposomes.