公开(公告)号：GR77539B

公开(公告)日：1984-09-24

申请号：GR830171606

申请日：1983-06-09

Applicant: HOECHST AG

Inventor： WINNACKER ERNST LUDWIG PROF DR ,  ESSER KARL PROF DR ,  PRAVE PAUL PROF DR ,  STAHL ULF DR ,  MARQUAROT RUDIGER DR ,  WOHNER GERHARD DR

IPC: C12N1/20 ,  C12N15/00 ,  C12N15/74 ,  C12P21/02

公开(公告)号：DE3119196A1

公开(公告)日：1982-12-09

申请号：DE3119196

申请日：1981-05-14

Applicant: HOECHST AG

Inventor： MINUTH WALTER ,  ESSER KARL PROF DR ,  MRACEK MIROSLAV ,  NESEMANN GEORG DR

IPC: C12N15/02 ,  C12N1/14 ,  C12N15/04 ,  C12N15/00 ,  C12P1/02

公开(公告)号：DE3380357D1

公开(公告)日：1989-09-14

申请号：DE3380357

申请日：1983-06-07

Applicant: HOECHST AG

Inventor： WINNACKER ERNST LUDWIG PROF DR ,  ESSER KARL PROF DR ,  PRAVE PAUL PROF DR ,  STAHL ULF DR ,  MARQUARDT RUDIGER DR ,  WOHNER GERHARD DR

IPC: C12N1/20 ,  C12N15/00 ,  C12N15/74 ,  C12P21/02

公开(公告)号：DE3222142A1

公开(公告)日：1983-12-15

申请号：DE3222142

申请日：1982-06-11

Applicant: HOECHST AG

Inventor： WINNACKER ERNST LUDWIG PROF ,  ESSER KARL PROF DR ,  PRAEVE PAUL PROF DR ,  STAHL ULF DR ,  MARQUARDT RUEDIGER DIPL CHEM ,  WOEHNER GERHARD DR

IPC: C12N15/09 ,  C07H21/04 ,  C07K14/62 ,  C12N1/20 ,  C12N15/00 ,  C12P21/02

Abstract: A hybrid plasmid with the replicon intrinsic to the methylotrophic bacterium is obtained from a plasmid from obligately methylotrophic bacteria, e.g. Methylomonas, on the one hand and a plasmid with selection markers on the other hand. This hybrid plasmid is - where appropriate after previous insertion of further genetic information - introduced by transformation into a host organism such as E. coli. After amplification and appropriate selection, the clones are treated with a suitable conjugative plasmid, and the mobilisability defect is eliminated by introducing another suitable plasmid. The clones obtained in this way are conjugated with plasmid-free obligately methylotrophic bateria as recipient, resulting - after selection - in clones which express proteins in accordance with the inserted genetic information.

公开(公告)号：AT45381T

公开(公告)日：1989-08-15

申请号：AT83105569

申请日：1983-06-07

Applicant: HOECHST AG

Inventor： WINNACKER ERNST LUDWIG PROF DR ,  ESSER KARL PROF DR ,  PRAEVE PAUL PROF DR ,  STAHL ULF DR ,  MARQUARDT RUEDIGER DR ,  WOEHNER GERHARD DR

IPC: C12N1/20 ,  C12N15/00 ,  C12P21/02

公开(公告)号：DE3121815A1

公开(公告)日：1983-02-24

申请号：DE3121815

申请日：1981-06-02

Applicant: HOECHST AG

Inventor： ESSER KARL PROF DR ,  STAHL ULF DIPL ING DR RER NAT ,  TUDZYNSKI PAUL DR RER NAT ,  KUECK ULRICH

IPC: C12N15/09 ,  C07H21/04 ,  C12N15/68 ,  C12N15/80 ,  C12N15/00

Abstract: What are disclosed are a hybrid vector synthesized from a segment of mitochondrial DNA containing an origin of replication and, optionally, from a segment of a prokaryotic plasmid, and a method for improving the amplification and expression of hybrid vectors in eukaryotic host cells using mitochondrial DNA.