公开(公告)号：DE3743824C2

公开(公告)日：1997-03-06

申请号：DE3743824

申请日：1987-12-23

Applicant: HOECHST AG

Inventor： KELLER REINHOLD DR ,  HOLLA WOLFGANG DR ,  FUELLING GERD DR

IPC: A61K31/045 ,  C12P7/62 ,  C12P41/00

Abstract: Racemic alcohols can be resolved in good yield and with high enantiomeric purity in the presence of vinyl esters with the aid of lipases from pig pancreas, pig liver and microorganisms.

公开(公告)号：DE3903446A1

公开(公告)日：1990-10-18

申请号：DE3903446

申请日：1989-02-06

Applicant: HOECHST AG

Inventor： WILLMS LOTHAR DR ,  FUELLING GERD DR ,  KELLER REINHOLD DR

IPC: C07F9/30 ,  C07F9/32 ,  C12P13/02 ,  C12P13/04 ,  C12P41/00

Abstract: L-PTC (L-phosphinothricin, L-2-amino-4-methylphosphinobutyric acid) is the active herbicidal component in D,L-PTC and can be obtained according to the invention by treating D,L-PTC derivatives which are N-acylated and esterified on the phosphinic acid group, as well as optionally esterified or amidated on the carboxyl group, with an enzyme with hydrolytic activity in aqueous or aqueous-organic medium, whereupon the L-PTC derivatives are selectively hydrolysed at the N-acyl group or the modified carboxyl group, the resulting product mixture is separated, and the required L-PTC derivative is hydrolysed to L-PTC and isolated by customary methods.

公开(公告)号：DE3743824A1

公开(公告)日：1989-07-06

申请号：DE3743824

申请日：1987-12-23

Applicant: HOECHST AG

Inventor： KELLER REINHOLD DR ,  HOLLA WOLFGANG DR ,  FUELLING GERD DR

IPC: A61K31/045 ,  C12P7/62 ,  C12P41/00

Abstract: Racemic alcohols can be resolved in good yield and with high enantiomeric purity in the presence of vinyl esters with the aid of lipases from pig pancreas, pig liver and microorganisms.

公开(公告)号：DE3622662A1

公开(公告)日：1988-01-14

申请号：DE3622662

申请日：1986-07-05

Applicant: HOECHST AG

Inventor： MUELLER WOLFGANG DR ,  KELLER REINHOLD DR ,  WULLBRANDT DIETER DR

IPC: C12P41/00 ,  C12M1/12 ,  C12M1/40 ,  C12P1/00 ,  C12P13/00 ,  C12P13/04 ,  C12N9/48 ,  C12N9/76 ,  C12N9/80 ,  C12P13/14 ,  C12P13/20 ,  C12P13/22

公开(公告)号：DE3466893D1

公开(公告)日：1987-11-26

申请号：DE3466893

申请日：1984-09-20

Applicant: HOECHST AG

Inventor： GRABLEY SUSANNE DR ,  KELLER REINHOLD DR ,  SCHLINGMANN MERTEN DR

IPC: C12N9/48 ,  C12N11/02 ,  C12N11/06 ,  C12N11/08 ,  C12P13/04 ,  C12P37/06 ,  C12P41/00

Abstract: 1. A process for the preparation of an immobilized acylase, wherein the enzyme is bound by means of a diisocyanate to a macroporous anion exchange resin based on phenol/formaldehyde and carrying essentially secondary amino groups.

公开(公告)号：AT28897T

公开(公告)日：1987-08-15

申请号：AT84111209

申请日：1984-09-20

Applicant: HOECHST AG

Inventor： KELLER REINHOLD DR ,  SCHLINGMANN MERTEN DR

IPC: C12N9/16 ,  C12N11/02 ,  C12N11/06 ,  C12N11/08 ,  C12P19/30

公开(公告)号：AT28751T

公开(公告)日：1987-08-15

申请号：AT84102462

申请日：1984-03-08

Applicant: HOECHST AG

Inventor： KELLER REINHOLD DR ,  SCHLINGMANN MERTEN DR

IPC: C12P19/34 ,  C07H1/08 ,  C07H21/00 ,  C07H21/02 ,  C12N15/10 ,  C12Q1/68 ,  C12Q1/6806 ,  B01D13/01

公开(公告)号：DE3334846A1

公开(公告)日：1985-04-04

申请号：DE3334846

申请日：1983-09-27

Applicant: HOECHST AG

Inventor： GRABLEY SUSANNE DR ,  KELLER REINHOLD DR ,  SCHLINGMANN MERTEN DR

IPC: C12N9/48 ,  C12N11/02 ,  C12N11/06 ,  C12N11/08 ,  C12P13/04 ,  C12P37/06 ,  C12P41/00 ,  C12N9/78 ,  C12N9/84 ,  C12P13/06 ,  C07F9/50 ,  C07C101/04

Abstract: 1. A process for the preparation of an immobilized acylase, wherein the enzyme is bound by means of a diisocyanate to a macroporous anion exchange resin based on phenol/formaldehyde and carrying essentially secondary amino groups.

公开(公告)号：DE3136940A1

公开(公告)日：1983-03-31

申请号：DE3136940

申请日：1981-09-17

Applicant: HOECHST AG

Inventor： KELLER REINHOLD DR ,  SCHLINGMANN MERTEN DR

IPC: C12P19/32 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C12N11/08 ,  C12N11/12 ,  C12P19/30 ,  C07H21/02 ,  C07H21/04

Abstract: Ribonucleic acid in a mixture of crude nucleic acids is selectively hydrolyzed with 5'-phosphodiesterase to 5'-ribonucleotides without hydrolyzing desoxyribonucleic acid in the mixture. Selective hydrolysis is carrying out by contacting the crude nucleic acid mixture with 5'-phosphodiesterase immobilized on a polymer carrier. The crude mixture of nucleic acids is preferably obtained by aqueous extraction of microorganisms that have previously been extracted with ammonia and a lower alcohol to remove lipids. The polymer carrier is preferably a copolymer of glycidylmethacrylate, allylglycidylether, methacrylamide and methylene-bis-methacrylamide.

公开(公告)号：DE4336322A1

公开(公告)日：1995-04-27

申请号：DE4336322

申请日：1993-10-25

Applicant: HOECHST AG

Inventor： RUBROEDER FRANZ-JOSEF DIPL ING ,  KELLER REINHOLD DR

IPC: C07K14/62 ,  C07K19/00 ,  C12N15/62 ,  C07K1/30

Abstract: The present invention relates to a method for increasing the solubility of fusion proteins of low solubility by treatment with an organic acid or with mixtures of a plurality of acids.