公开(公告)号：AU2005263591A1

公开(公告)日：2006-01-26

申请号：AU2005263591

申请日：2005-07-19

Applicant: HOFFMANN LA ROCHE

Inventor： LAGGERBAUER JESSICA ,  KNAPPE WOLFGANG-REINHOLD ,  BOENITZ-DULAT MARA ,  SCHMUCK RAINER ,  KRATZSCH PETER

IPC: C12N9/04 ,  C12M1/40 ,  C12N15/10 ,  C12N15/53 ,  C12Q1/32 ,  G01N33/66

Abstract: The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known fromto genes encoding such variant s-GDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.

公开(公告)号：NO340499B1

公开(公告)日：2017-05-02

申请号：NO20070946

申请日：2007-02-19

Applicant: HOFFMANN LA ROCHE

Inventor： DULAT MARA BOENITZ ,  KNAPPE WOLFGANG-REINHOLD ,  KRATZSCH PETER ,  LAGGERBAUER JESSICA ,  SCHMUCK RAINER

IPC: C12N9/04 ,  C12N5/12 ,  C12N15/11 ,  C12N15/52 ,  C12N15/63

公开(公告)号：AU2005263591B2

公开(公告)日：2008-08-14

申请号：AU2005263591

申请日：2005-07-19

Applicant: HOFFMANN LA ROCHE

Inventor： LAGGERBAUER JESSICA ,  KNAPPE WOLFGANG-REINHOLD ,  BOENITZ-DULAT MARA ,  SCHMUCK RAINER ,  KRATZSCH PETER

IPC: C12N9/04 ,  C12M1/40 ,  C12N15/10 ,  C12N15/53 ,  C12Q1/32 ,  G01N33/66

Abstract: The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known fromto genes encoding such variant s-GDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.

公开(公告)号：CA2570593C

公开(公告)日：2012-03-20

申请号：CA2570593

申请日：2005-07-19

Applicant: HOFFMANN LA ROCHE

Inventor： BOENITZ-DULAT MARA ,  LAGGERBAUER JESSICA ,  SCHMUCK RAINER ,  KRATZSCH PETER ,  KNAPPE WOLFGANG-REINHOLD

IPC: C12N9/04 ,  C12M1/40 ,  C12N15/10 ,  C12N15/53 ,  C12Q1/32 ,  G01N33/66

Abstract: The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known from Acinetobacter calcoaceticus, to genes encoding such variant s~GDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.

公开(公告)号：NZ551870A

公开(公告)日：2009-02-28

申请号：NZ55187005

申请日：2005-07-19

Applicant: HOFFMANN LA ROCHE

Inventor： BOENITZ-DULAT MARA ,  LAGGERBAUER JESSICA ,  SCHMUCK RAINER ,  KRATZSCH PETER ,  KNAPPE WOLFGANG-REINHOLD

IPC: C12M1/40 ,  C12N15/10 ,  C12N15/53 ,  C12N9/04 ,  C12Q1/32 ,  G01N33/66

Abstract: Provided is a variant of PQQ-dependent soluble glucose dehydrogenase polypeptide comprising one amino acid insertion at position 428-428 of the wild type sequence from Acinetobacter calcoaceticus. Further provided are specific variants with specified amino acids in that position, and with further amino acid insertions in other specified positions. Also provided are corresponding polynucleotides, vectors coding for the variant, cells containing the vector, methods of producing the variants. The compositions and methods are useful for determining concentrations of sugars in a sample.

公开(公告)号：HK1107829A1

公开(公告)日：2008-04-18

申请号：HK07113450

申请日：2007-12-10

Applicant: HOFFMANN LA ROCHE

Inventor： BOENITZ-DULAT MARA ,  LAGGERBAUER JESSICA ,  SCHMUCK RAINER ,  KRATZSCH PETER ,  KNAPPE WOLFGANG-REINHOLD

IPC: C12M20100101 ,  C12N20100101 ,  C12Q20100101 ,  G01N20100101

Abstract: The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known fromto genes encoding such variant s-GDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.

公开(公告)号：BRPI0513691A

公开(公告)日：2008-03-25

申请号：BRPI0513691

申请日：2005-07-19

Applicant: ROCHE DIAGNOSTICS GMBH ,  HOFFMANN LA ROCHE

Inventor： BOENITZ-DULAT MARA ,  LAGGERBAUER JESSICA ,  SCHMUCK RAINER ,  KRATZSCH PETER ,  KNAPPE WOLFGANG-REINHOLD

IPC: C12N9/04 ,  C12M1/40 ,  C12N15/10 ,  C12N15/53 ,  C12Q1/32 ,  G01N33/66

Abstract: The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known fromto genes encoding such variant s-GDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.

公开(公告)号：NO20070946L

公开(公告)日：2007-04-11

申请号：NO20070946

申请日：2007-02-19

Applicant: HOFFMANN LA ROCHE

Inventor： DULAT MARA BOENITZ ,  KNAPPE WOLFGANG-REINHOLD ,  KRATZSCH PETER ,  LAGGERBAUER JESSICA ,  SCHMUCK RAINER

IPC: C12N9/04 ,  C12N15/10 ,  C12N15/53 ,  C12Q1/32 ,  G01N33/66

Abstract: The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known fromto genes encoding such variant s-GDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.

公开(公告)号：MX2007000560A

公开(公告)日：2007-03-07

申请号：MX2007000560

申请日：2005-07-19

Applicant: HOFFMANN LA ROCHE

Inventor： SCHMUCK RAINER ,  KNAPPE WOLFGANG-REINHOLD ,  BOENITZ-DULAT MARA ,  KRATZSCH PETER ,  LAGGERBAUER JESSICA

IPC: C12M1/00 ,  C12N9/00 ,  C12N15/00 ,  C12Q1/00 ,  G01N33/00

Abstract: La presente invencion se refiere a variantes mejoradas de glucosa-deshidrogenasas dependientes de pirroloquinolin-quinona (PQQ) solubles (s-GDH), que comprenden una insercion de aminoacidos entre las posiciones 428 y 429 como corresponde a la secuencia de aminoacidos conocida de Acinetobacter calcoaceticus, a genes que codifican tales variantes de s-GDH, a las proteinas de tales variantes de s-GDH con especificidad mejorada de sustrato para glucosa, y a diferentes aplicaciones de estas variantes de s-GDH, particularmente para determinar concentraciones de azucares, especialmente de glucosa en una muestra.

公开(公告)号：CA2570593A1

公开(公告)日：2006-01-26

申请号：CA2570593

申请日：2005-07-19

Applicant: HOFFMANN LA ROCHE

Inventor： KNAPPE WOLFGANG-REINHOLD ,  KRATZSCH PETER ,  SCHMUCK RAINER ,  LAGGERBAUER JESSICA ,  BOENITZ-DULAT MARA

IPC: C12N9/04 ,  C12M1/40 ,  C12N15/10 ,  C12N15/53 ,  C12Q1/32 ,  G01N33/66

Abstract: The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known fromto genes encoding such variant s-GDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.