公开(公告)号：US09448175B2

公开(公告)日：2016-09-20

申请号：US14423030

申请日：2013-08-19

Applicant: HORIBA ABX SAS

Inventor： Alexandra Urankar ,  Philippe Nerin ,  Moustapha Hariche ,  Gilles Cauet

IPC: G01N21/64 ,  G01N21/27 ,  G01N15/14 ,  G01N33/49 ,  G01N35/00 ,  G01N15/10

CPC classification number: G01N21/6486 ,  G01N15/147 ,  G01N21/274 ,  G01N21/6428 ,  G01N33/49 ,  G01N35/00663 ,  G01N2015/1006 ,  G01N2035/00673 ,  G01N2201/121

Abstract: The present invention relates to a method for compensating for the breakdown of a reagent stored in an aqueous phase comprising at least one fluorescent compound and enabling the identification of particles, including the steps of: (i) measuring the fluorescence level FLUOm(t) of particles marked with said reagent; (ii) measuring the absorbance at at least one wavelength of a solution of said reagent, at a time t close to the time of said fluorescence level FLUOm(t) measurements, so as to determine at least one current optical density DO(t) of the reagent; and (iii) calculating a correction of the fluorescent level measurements using said at least one current optical density DO(t) and at least one initial optical density DO(0) of the reagent that has not been broken down. The invention also relates to a biological analysis device implementing the method.

Abstract translation: 本发明涉及一种用于补偿存储在包含至少一种荧光化合物的水相中的试剂的分解的方法，并且能够鉴定颗粒，包括以下步骤：（i）测量荧光水平FLUOm（t） 用所述试剂标记的颗粒; （ii）在接近所述荧光水平FLUOm（t）测量时间的时间t测量所述试剂溶液的至少一个波长处的吸光度，以便确定至少一个当前光密度DO（t） 的试剂; 以及（iii）使用所述至少一个当前光密度DO（t）和至少一个未被分解的试剂的初始光密度DO（0）来计算荧光水平测量的校正。 本发明还涉及实现该方法的生物分析装置。

公开(公告)号：US20150241352A1

公开(公告)日：2015-08-27

申请号：US14423030

申请日：2013-08-19

Applicant: HORIBA ABX SAS

Inventor： Alexandra Urankar ,  Philippe Nerin ,  Moustapha Hariche ,  Gilles Cauet

IPC: G01N21/64 ,  G01N33/49

CPC classification number: G01N21/6486 ,  G01N15/147 ,  G01N21/274 ,  G01N21/6428 ,  G01N33/49 ,  G01N35/00663 ,  G01N2015/1006 ,  G01N2035/00673 ,  G01N2201/121

Abstract: The present invention relates to a method for compensating for the breakdown of a reagent stored in an aqueous phase comprising at least one fluorescent compound and enabling the identification of particles, including the steps of: (i) measuring the fluorescence level FLUOm(t) of particles marked with said reagent; (ii) measuring the absorbance at at least one wavelength of a solution of said reagent, at a time t close to the time of said fluorescence level FLUOm(t) measurements, so as to determine at least one current optical density DO(t) of the reagent; and (iii) calculating a correction of the fluorescent level measurements using said at least one current optical density DO(t) and at least one initial optical density DO(0) of the reagent that has not been broken down. The invention also relates to a biological analysis device implementing the method.

Abstract translation: 本发明涉及一种用于补偿存储在包含至少一种荧光化合物的水相中的试剂的分解的方法，并且能够鉴定颗粒，包括以下步骤：（i）测量荧光水平FLUOm（t） 用所述试剂标记的颗粒; （ii）在接近所述荧光水平FLUOm（t）测量时间的时间t测量所述试剂溶液的至少一个波长处的吸光度，以便确定至少一个当前光密度DO（t） 的试剂; 以及（iii）使用所述至少一个当前光密度DO（t）和至少一个未被分解的试剂的初始光密度DO（0）来计算荧光水平测量的校正。 本发明还涉及实现该方法的生物分析装置。