公开(公告)号：JPH03103193A

公开(公告)日：1991-04-30

申请号：JP4914090

申请日：1990-02-28

Applicant: HOECHST AG

Inventor： ETSUKUHARUTO SHIYUTORAUHO ,  UORUFUGANGU UOORUREEBEN ,  WARUTAA ARUNORUTO ,  RENAATE ARIYAA ,  ARUFUREETO PIYUURAA ,  GERUHARUTO UENAA ,  RIYUUDEIGAA MARUKUBARUTO ,  ZUZANNE GURAAPURAI ,  DEIITAA BURAUAA ,  KURAUSU BAARUCHIE

IPC: C12N15/09 ,  C12N5/10 ,  C12N9/10 ,  C12N15/00 ,  C12N15/54 ,  C12N15/64 ,  C12N15/82 ,  C12P41/00 ,  C12Q1/68 ,  C12R1/465

公开(公告)号：JPS645493A

公开(公告)日：1989-01-10

申请号：JP11804388

申请日：1988-05-14

Applicant: HOECHST AG

Inventor： DEIITAA BURAUAA ,  KURAUSU BARUCHIYU ,  GIYUNTAA DON

IPC: A01H5/00 ,  A01N63/00 ,  C02F3/34 ,  C12N9/10 ,  C12N15/00 ,  C12N15/09 ,  C12N15/31 ,  C12N15/54 ,  C12N15/82 ,  C12R1/01 ,  C12R1/05 ,  C12R1/38 ,  C12R1/425

公开(公告)号：JPH09107981A

公开(公告)日：1997-04-28

申请号：JP26891096

申请日：1996-10-09

Applicant: HOECHST AG

Inventor： ETSUKUHARUTO SHIYUTORAUHO ,  UORUFUGANGU UOORUREEBEN ,  WARUTAA ARUNORUTO ,  RENAATE ARIYAA ,  ARUFUREETO PIYUURAA ,  GERUHARUTO UEENAA ,  RIYUUDEIGAA MARUKUBARUTO ,  ZUZANNE GURAAPURAI ,  DEIITAA BURAUAA ,  KURAUSU BAARUCHIYU

IPC: C12N15/09 ,  C12N5/10 ,  C12N9/10 ,  C12N15/00 ,  C12N15/54 ,  C12N15/64 ,  C12N15/82 ,  C12P41/00 ,  C12Q1/68 ,  C12R1/465

公开(公告)号：JPS6147198A

公开(公告)日：1986-03-07

申请号：JP17440485

申请日：1985-08-09

Applicant: Hoechst Ag

Inventor： DEIITAA BURAUAA ,  YOOAHIMU ENGERUSU ,  PAURU HAABERUMAN ,  OIGEEN UURUMAN ,  FURIIDORIHI BUENGENMAIAA

IPC: C12N15/09 ,  A61K38/00 ,  C07H21/00 ,  C07H21/04 ,  C07K14/00 ,  C07K14/435 ,  C07K14/815 ,  C12N1/20 ,  C12N15/00 ,  C12N15/72 ,  C12P21/02 ,  C12R1/19

CPC classification number: C07H21/00 ,  A61K38/00 ,  C07K14/815 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/036 ,  C07K2319/75 ,  C12N15/72

Abstract: Hirudin can be obtained using a specific DNA sequence in a genetic engineering process. The gene is advantageously synthesised in the form of several fragments which are enzymatically ligated to give two larger part-sequences which are incorporated into hybrid plasmids and amplified in host organisms. The part-sequences are reisolated and then combined to form the complete gene which is incorporated into a hybrid plasmid and the latter is expressed in a host organism.

公开(公告)号：JPH07147985A

公开(公告)日：1995-06-13

申请号：JP24962794

申请日：1994-10-14

Applicant: HOECHST AG

Inventor： ETSUKUHARUTO SHIYUTORAUHO ,  UORUFUGANGU UOORUREEBEN ,  WARUTAA ARUNORUTO ,  RENAATE ARIYAA ,  ARUFUREETO PIYUURAA ,  GERUHARUTO UEENAA ,  RIYUUDEIGAA MARUKUBARUTO ,  ZUZANNE GURAAPURAI ,  DEIITAA BURAUAA ,  KURAUSU BAARUCHIYU

IPC: C12N15/09 ,  C12N5/10 ,  C12N9/10 ,  C12N15/00 ,  C12N15/54 ,  C12N15/64 ,  C12N15/82 ,  C12P41/00 ,  C12Q1/68 ,  C12R1/465

公开(公告)号：JPS6371183A

公开(公告)日：1988-03-31

申请号：JP20912387

申请日：1987-08-22

Applicant: HOECHST AG

Inventor： ETSUKUHARUTO SHIYUTORAUHO ,  UORUFUGANGU UOORUREEBEN ,  WARUTAA ARUNORUTO ,  RENAATE ARIYAA ,  ARUFUREETO PIYUURAA ,  GERUHARUTO UEENAA ,  RIYUUDEIGAA MARUKUBARUTO ,  ZUZANNE GURAAPURAI ,  DEIITAA BURAUAA ,  KURAUSU BAARUCHIYU

IPC: C12N15/09 ,  C12N5/10 ,  C12N9/10 ,  C12N15/00 ,  C12N15/54 ,  C12N15/64 ,  C12N15/82 ,  C12P41/00 ,  C12Q1/68 ,  C12R1/465