公开(公告)号：WO1998028075A1

公开(公告)日：1998-07-02

申请号：PCT/IB1997001585

申请日：1997-12-18

Applicant: IMAGING RESEARCH INC. ,  RAMM, Peter

Inventor： IMAGING RESEARCH INC.

IPC: B01L03/00

CPC classification number: G01N21/03 ,  B01J2219/00317 ,  B01L3/5085 ,  B01L2300/0893 ,  C40B60/14 ,  G01N21/251 ,  G01N21/64 ,  G01N21/76 ,  G01N2021/0346 ,  G01N2021/0378

Abstract: A specimen containment system is disclosed which optimizes the optical properties of the specimen holders (wells) for imaging in order to enhance the relevant signal, relative to background. A family of well plates all have the inherent properties of: i) minimizing reflections from an excitation source into an imaging detector; ii) minimizing the concentration of excitation into intense and localized sources (lensing); and iii) allowing an epi-illuminating excitation beam to penetrate both central and lateral wells effectively. The well plates are made of a material that has or is treated to have minimal inherent fluorescence and minimal reflection properties. The well chambers are constructed to be depth-wise asymmetric (for example, sloping well surfaces) and to have cross-sectional irregularity. The bottom walls of the wells may also be formed with a ridge or made transparent so as to minimize reflections of incident light therefrom.

Abstract translation: 公开了一种样品容纳系统，其优化用于成像的样品保持器（孔）的光学性质，以便相对于背景增强相关信号。 一系列井板都具有固有的特性：i）最小化从激发源到成像探测器的反射; ii）将激发的浓度最小化为强烈和局部源（透镜）; 和iii）允许外照射激发光束有效地穿透中心阱和侧向阱。 孔板由具有或被处理为具有最小固有荧光和最小反射性质的材料制成。 井室被构造为深度不对称的（例如，倾斜的井表面）并且具有横截面的不规则性。 孔的底壁也可以形成有脊或制成透明的，以便使来自其的入射光的反射最小化。