公开(公告)号：JPH06508987A

公开(公告)日：1994-10-13

申请号：JP51004692

申请日：1992-04-09

Applicant: INDIANA UNIVERSITY FOUNDATION

Inventor： HOFFMAN RONALD ,  BRANDT JOHN

IPC: C12N5/0789 ,  C12N5/08

公开(公告)号：CA2141207A1

公开(公告)日：1994-02-03

申请号：CA2141207

申请日：1993-07-27

Applicant: INDIANA UNIVERSITY FOUNDATION

Inventor： SROUR EDWARD F ,  BRANDT JOHN E ,  ZANJANI ESMAIL D ,  HOFFMAN RONALD

IPC: A61K35/12 ,  A61K35/28 ,  C12N5/0789 ,  C12N5/00 ,  A61K48/00

Abstract: 2141207 9402157 PCTABS00030 In the field of hematopoietic stem cell isolation and implantation or engraftment, methods are disclosed for isolating cell populations that are highly enriched for human pluripotent hematopoietic stem cells. The cells are CD34+, HLA-DR- and express the receptor for the c-kit ligand (KR+). Methods of growing the cells in long term bone marrow cultures in the presence of c-kit ligand and other cytokines are disclosed. The cells may be useful for transplantation and for use in gene therapy protocols.

公开(公告)号：AU4788093A

公开(公告)日：1994-02-14

申请号：AU4788093

申请日：1993-07-27

Applicant: INDIANA UNIVERSITY FOUNDATION

Inventor： SROUR EDWARD F ,  ZANJANI ESMAIL D ,  BRANDT JOHN E ,  HOFFMAN RONALD

IPC: A61K35/12 ,  A61K35/28 ,  C12N5/0789 ,  C12N5/02 ,  C12N5/08

Abstract: Disclosed are human pluripotent hematopoietic stem cell (PHSC) enriched compositions and methods for obtaining and using the compositions. A substantially homogeneous population of human hematopoietic cells characterized as CD34+, HLA-DR-, and c-kit receptor positive (KR+) and capable of in vitro self-renewal and differentiation to members of at least the erythroid, myeloid, and megakaryocytic lineages, is obtained by separating the population from a cellular mixture, for example by cytometric cell sorting techniques. Also disclosed is a method of obtaining persistent maintenance of grafted human hematopoietic cells in a mammal, which includes grafting a mammal in utero with a cellular composition enriched in human hematopoietic cells characterized as CD34+, HLA-DR- and capable of in vitro self-renewal and differentiation to members of the lymphoid, myeloid, erythroid and megakaryocytic lineages.

公开(公告)号：CA2107897A1

公开(公告)日：1992-10-10

申请号：CA2107897

申请日：1992-04-09

Applicant: INDIANA UNIVERSITY FOUNDATION

Inventor： HOFFMAN RONALD ,  BRANDT JOHN

IPC: C12N5/0789 ,  C12N5/08 ,  C12N5/02 ,  C12N5/06 ,  A61K35/28

Abstract: 2107897 9218615 PCTABS00017 A process for supporting hematopoietic progenitor cells in a culture medium which contains at least one cytokine effective for supporting the cells, and preferably, is essentially free of stromal cells.

公开(公告)号：EP0584184A4

公开(公告)日：1995-12-27

申请号：EP92910694

申请日：1992-04-09

Applicant: INDIANA UNIVERSITY FOUNDATION

Inventor： HOFFMAN RONALD ,  BRANDT JOHN

IPC: C12N5/0789 ,  C12N5/00

CPC classification number: C12N5/0647 ,  C12N2500/90 ,  C12N2501/125 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/39

Abstract: A process for supporting hematopoietic progenitor cells in a culture medium which contains at least one cytokine effective for supporting the cells, and preferably, is essentially free of stromal cells.

公开(公告)号：EP0658114A4

公开(公告)日：1996-07-10

申请号：EP93918427

申请日：1993-07-27

Applicant: INDIANA UNIVERSITY FOUNDATION

Inventor： SROUR EDWARD F ,  ZANJANI ESMAIL D ,  BRANDT JOHN E ,  HOFFMAN RONALD

IPC: A61K35/12 ,  A61K35/28 ,  C12N5/0789 ,  C12N5/02 ,  C12N5/08

CPC classification number: C12N5/0647 ,  A61K35/28 ,  A61K2035/124 ,  C07K2319/00 ,  C07K2319/75 ,  C12N2501/125 ,  C12N2501/14 ,  C12N2501/22 ,  C12N2501/23

Abstract: Disclosed are human pluripotent hematopoietic stem cell (PHSC) enriched compositions and methods for obtaining and using the compositions. A substantially homogeneous population of human hematopoietic cells characterized as CD34+, HLA-DR-, and c-kit receptor positive (KR+) and capable of in vitro self-renewal and differentiation to members of at least the erythroid, myeloid, and megakaryocytic lineages, is obtained by separating the population from a cellular mixture, for example by cytometric cell sorting techniques. Also disclosed is a method of obtaining persistent maintenance of grafted human hematopoietic cells in a mammal, which includes grafting a mammal in utero with a cellular composition enriched in human hematopoietic cells characterized as CD34+, HLA-DR- and capable of in vitro self-renewal and differentiation to members of the lymphoid, myeloid, erythroid and megakaryocytic lineages.