公开(公告)号：CA2093804A1

公开(公告)日：1992-04-12

申请号：CA2093804

申请日：1991-10-11

Applicant: INDIANA UNIVERSITY FOUNDATION

Inventor： SURZYCKI STEFAN J ,  KITYAMA MASAHIKO ,  TOGASAKI ROBERT K

IPC: B02C19/00 ,  A61M11/06 ,  B05B7/00 ,  C12M1/00 ,  C12M1/33 ,  C12M3/08 ,  C12Q1/68 ,  G01N1/00 ,  G01N1/28 ,  G01N1/40

Abstract: Described is an improved process for fragmenting a biomaterial and isolating and recovering a component thereof. The preferred improved process includes the step of performing the fragmentation by nebulizing a liquid medium containing the bi- omaterial. A preferred process for fragmenting isolated DNA includes the ste p of nebulizing a fluid containing the DNA. This preferred process provides randomness superior to prior known DNA fragmentation methods, as well as other important advan- tages. An improved nebulization device is also described.

公开(公告)号：GR3033062T3

公开(公告)日：2000-08-31

申请号：GR20000400750

申请日：2000-03-27

Applicant: INDIANA UNIVERSITY FOUNDATION

Inventor： SURZYCKI STEFAN J ,  KITYAMA MASAHIKO ,  TOGASAKI ROBERT K

IPC: B02C19/00 ,  A61M11/06 ,  B05B7/00 ,  C12M1/00 ,  C12M1/33 ,  C12M3/08 ,  C12Q1/68 ,  G01N1/00 ,  G01N1/28 ,  G01N1/40 ,  C07H15/12 ,  C12P19/34

Abstract: PCT No. PCT/US91/07530 Sec. 371 Date Mar. 7, 1994 Sec. 102(e) Date Mar. 7, 1994 PCT Filed Oct. 11, 1991 PCT Pub. No. WO92/07091 PCT Pub. Date Apr. 30, 1992Described is an improved process for fragmenting a biomaterial and isolating and recovering a component thereof. The preferred improved process includes the step of performing the fragmentation by nebulizing a liquid medium containing the biomaterial. A preferred process for fragmenting isolated DNA includes the step of nebulizing a fluid containing the DNA. This preferred process provides randomness superior to prior known DNA fragmentation methods, as well as other important advantages. An improved nebulization device is also described.

公开(公告)号：AU667293B2

公开(公告)日：1996-03-21

申请号：AU8903691

申请日：1991-10-11

Applicant: INDIANA UNIVERSITY FOUNDATION

Inventor： SURZYCKI STEFAN J ,  KITYAMA MASAHIKO ,  TOGASAKI ROBERT K

IPC: B02C19/00 ,  A61M11/06 ,  B05B7/00 ,  C12M1/00 ,  C12M1/33 ,  C12M3/08 ,  C12Q1/68 ,  G01N1/00 ,  G01N1/28 ,  G01N1/40 ,  C07K3/02

Abstract: PCT No. PCT/US91/07530 Sec. 371 Date Mar. 7, 1994 Sec. 102(e) Date Mar. 7, 1994 PCT Filed Oct. 11, 1991 PCT Pub. No. WO92/07091 PCT Pub. Date Apr. 30, 1992Described is an improved process for fragmenting a biomaterial and isolating and recovering a component thereof. The preferred improved process includes the step of performing the fragmentation by nebulizing a liquid medium containing the biomaterial. A preferred process for fragmenting isolated DNA includes the step of nebulizing a fluid containing the DNA. This preferred process provides randomness superior to prior known DNA fragmentation methods, as well as other important advantages. An improved nebulization device is also described.

公开(公告)号：AU8903691A

公开(公告)日：1992-05-20

申请号：AU8903691

申请日：1991-10-11

Applicant: INDIANA UNIVERSITY FOUNDATION

Inventor： SURZYCKI STEFAN J ,  KITYAMA MASAHIKO ,  TOGASAKI ROBERT K

IPC: B02C19/00 ,  A61M11/06 ,  B05B7/00 ,  C12M1/00 ,  C12M1/33 ,  C12M3/08 ,  C12Q1/68 ,  G01N1/00 ,  G01N1/28 ,  G01N1/40 ,  C12P19/34 ,  C07H15/12

Abstract: PCT No. PCT/US91/07530 Sec. 371 Date Mar. 7, 1994 Sec. 102(e) Date Mar. 7, 1994 PCT Filed Oct. 11, 1991 PCT Pub. No. WO92/07091 PCT Pub. Date Apr. 30, 1992Described is an improved process for fragmenting a biomaterial and isolating and recovering a component thereof. The preferred improved process includes the step of performing the fragmentation by nebulizing a liquid medium containing the biomaterial. A preferred process for fragmenting isolated DNA includes the step of nebulizing a fluid containing the DNA. This preferred process provides randomness superior to prior known DNA fragmentation methods, as well as other important advantages. An improved nebulization device is also described.

公开(公告)号：EP0552290A4

公开(公告)日：1996-05-29

申请号：EP91919982

申请日：1991-10-11

Applicant: INDIANA UNIVERSITY FOUNDATION

Inventor： SURZYCKI STEFAN J ,  KITYAMA MASAHIKO ,  TOGASAKI ROBERT K

IPC: B02C19/00 ,  A61M11/06 ,  B05B7/00 ,  C12M1/00 ,  C12M1/33 ,  C12M3/08 ,  C12Q1/68 ,  G01N1/00 ,  G01N1/28 ,  G01N1/40 ,  C07H15/12 ,  C12P19/34

CPC classification number: G01N1/286 ,  B05B7/0012 ,  C12M47/06 ,  C12Q1/68 ,  G01N2001/4066

Abstract: Described is an improved process for fragmenting a biomaterial and isolating and recovering a component thereof. The preferred improved process includes the step of performing the fragmentation by nebulizing a liquid medium containing the biomaterial. A preferred process for fragmenting isolated DNA includes the step of nebulizing a fluid containing the DNA. This preferred process provides randomness superior to prior known DNA fragmentation methods, as well as other important advantages. An improved nebulization device is also described.