公开(公告)号：US10858662B2

公开(公告)日：2020-12-08

申请号：US15527837

申请日：2015-11-19

Applicant: INSTITUTE FOR BASIC SCIENCE

Inventor： Jin Soo Kim ,  Tae Young Koo

IPC: C12N15/74 ,  C12N15/63 ,  C12N15/11 ,  C12N15/864 ,  C12N15/867 ,  C12N9/16 ,  C12N9/22

Abstract: The present invention relates to a method for regulating gene expression, comprising introducing into a cell each of a recombinant vector which expresses a first domain comprising N-terminus of a Cas9 protein, and a recombinant vector which expresses a second domain comprising C-terminus of a Cas9 protein, a composition comprising the recombinant vectors, a kit for regulating gene expression, and a method for intracellular production of Cas9 protein. Moreover, the present invention relates to a transformed cell introduced with a viral vector which packages the first domain, and a viral vector which packages the second domain, and to a composition comprising a virus produced therefrom.

公开(公告)号：US20170349905A1

公开(公告)日：2017-12-07

申请号：US15527837

申请日：2015-11-19

Applicant: INSTITUTE FOR BASIC SCIENCE

Inventor： Jin Soo Kim ,  Tae Young Koo

IPC: C12N15/74 ,  C12N15/864 ,  C12N15/11 ,  C12N15/867 ,  C12N9/22

CPC classification number: C12N15/746 ,  C12N9/16 ,  C12N9/22 ,  C12N15/11 ,  C12N15/63 ,  C12N15/8645 ,  C12N15/867

Abstract: The present invention relates to a method for regulating gene expression, comprising introducing into a cell each of a recombinant vector which expresses a first domain comprising N-terminus of a Cas9 protein, and a recombinant vector which expresses a second domain comprising C-terminus of a Cas9 protein, a composition comprising the recombinant vectors, a kit for regulating gene expression, and a method for intracellular production of Cas9 protein. Moreover, the present invention relates to a transformed cell introduced with a viral vector which packages the first domain, and a viral vector which packages the second domain, and to a composition comprising a virus produced therefrom.