公开(公告)号：US10533193B2

公开(公告)日：2020-01-14

申请号：US14914741

申请日：2014-08-05

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Alex Van Eck Conradie ,  Adriana Leonora Botes

IPC: C12P5/02 ,  C12N1/21 ,  C12N9/88 ,  C12N9/12 ,  C12N9/16 ,  C12N9/10

Abstract: The document provides methods for biosynthesizing isobutene using one or more isolated enzymes such as one or more of a hydratase such as an enzyme classified under EC 4.2.1.- and a decarboxylating thioesterase, or using recombinant host cells expressing one or more such enzymes.

公开(公告)号：US10273518B2

公开(公告)日：2019-04-30

申请号：US14908659

申请日：2014-07-29

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Alex Van Eck Conradie ,  Adriana Leonora Botes

IPC: C12P19/32 ,  C12P7/40 ,  C12P7/26 ,  C12N9/10

Abstract: This document describes biochemical pathways that include the production of 3-oxopent-4-enoyl-CoA by condensation of acryloyl-CoA and acetyl-CoA using a β-ketothiolase with a SER-HIS-HIS catalytic triad. These pathways described herein rely on enzymes such as, inter alia, dehydrogenases, dehydratases and β-ketothiolases.

公开(公告)号：US10577634B2

公开(公告)日：2020-03-03

申请号：US15462975

申请日：2017-03-20

Applicant: INVISTA North America S.A.R.L.

Inventor： Paul S. Pearlman ,  Changlin Chen ,  Adriana L. Botes ,  Alex Van Eck Conradie ,  Benjamin D. Herzog

IPC: C12P7/44 ,  C12N9/02 ,  C12P13/04 ,  C12P17/10 ,  C08G69/16 ,  C08G69/14 ,  C08G69/26 ,  C08G69/28 ,  B29B9/16 ,  C12N9/04 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88 ,  C12N9/00 ,  C12P7/40 ,  C12P7/62 ,  C12P13/00 ,  C12P7/42 ,  C12P13/02 ,  C12N9/24

Abstract: Embodiments of the present invention relate to methods for the biosynthesis of di- or trifunctional C7 alkanes in the presence of isolated enzymes or in the presence of a recombinant host cell expressing those enzymes. The di- or trifunctional C7 alkanes are useful as intermediates in the production of nylon-7, nylon-7,x, nylon-x,7, and polyesters.

公开(公告)号：US20180002704A1

公开(公告)日：2018-01-04

申请号：US15637489

申请日：2017-06-29

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Achuthanunni Chokkathukalam ,  Alex Van Eck Conradie ,  Ramdane Haddouche ,  Satnam Surae ,  Katherine Louise Tibbles

IPC: C12N15/52 ,  C12P7/40 ,  C12N9/02 ,  C12N9/90 ,  C12N9/88 ,  C12N9/10 ,  C12N9/04 ,  C12N9/00

CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/13 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12P7/40 ,  C12Y101/01027 ,  C12Y101/99007 ,  C12Y102/01002 ,  C12Y102/0101 ,  C12Y102/01043 ,  C12Y103/01084 ,  C12Y203/01054 ,  C12Y203/03005 ,  C12Y208/03 ,  C12Y401/01031 ,  C12Y403/01006 ,  C12Y403/01019 ,  C12Y404/01001 ,  C12Y503/01027 ,  C12Y602/01036 ,  Y02P20/134

Abstract: The present disclosure relates to methods for more efficiently recycling reduced electron carriers in a hydrogen-oxidizing microorganism with an operable Calvin-Benson cycle; synthetic carbon fixation pathways that recycle reduced electron carriers more efficiently than the Calvin-Benson cycle, such as methods for enzymatically converting carbon dioxide to formate and assimilating the resulting formate into central carbon metabolism; methods for producing biochemical products; and recombinant hosts utilizing one or more synthetic carbon fixation pathways.

公开(公告)号：US20160355844A1

公开(公告)日：2016-12-08

申请号：US15213830

申请日：2016-07-19

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Paul S. Pearlman ,  Changlin Chen ,  Adriana Leonora Botes ,  Alex Van Eck Conradie

IPC: C12P5/02 ,  C12N15/52 ,  C12N9/88

CPC classification number: C12P5/026 ,  C12N9/001 ,  C12N9/0071 ,  C12N9/88 ,  C12N15/52 ,  C12P5/02 ,  C12P7/16 ,  C12P7/18 ,  C12Y103/01035 ,  C12Y103/08 ,  C12Y114/11022 ,  C12Y401/01033 ,  C12Y402/01 ,  C12Y402/01127 ,  C12Y402/03027 ,  Y02E50/10 ,  Y02E50/343 ,  Y02P20/52

Abstract: This document describes biochemical pathways for producing butadiene by forming two vinyl groups in a butadiene synthesis substrate. These pathways described herein rely on enzymes such as mevalonate diphosphate decarboxylase, isoprene synthase, and dehydratases for the final enzymatic step.

公开(公告)号：US20150079654A1

公开(公告)日：2015-03-19

申请号：US14334190

申请日：2014-07-17

Applicant: INVISTA North America S.a.r.l.

Inventor： Adriana Leonora Botes ,  Alex Van Eck Conradie

IPC: C12P5/02

CPC classification number: C12P5/026 ,  C12Y402/01127 ,  Y02E50/343

Abstract: This document describes biochemical pathways for producing butadiene by forming two vinyl groups in a butadiene synthesis substrate. These pathways described herein rely on enzymes such as, inter alia, a decarboxylating thioesterase, cytochrome P450, or dehydratases for the final enzymatic step.

Abstract translation: 该文献描述了通过在丁二烯合成底物中形成两个乙烯基来生产丁二烯的生化途径。 本文描述的这些途径依赖于酶，例如脱羧硫酯酶，细胞色素P450或用于最终酶促步骤的脱水酶。

公开(公告)号：US10538789B2

公开(公告)日：2020-01-21

申请号：US15808409

申请日：2017-11-09

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Mariusz Kamionka ,  Jan Modregger ,  Axel Niebisch ,  Alex Van Eck Conradie ,  Adriana Leonora Botes

IPC: C12P5/00 ,  C12N15/70 ,  C12P5/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N15/52

Abstract: This document describes biochemical pathways for producing isoprene by forming two vinyl groups in a central precursor produced from isobutyryl-CoA, 3-methyl-2-oxopentanoate, or 4-methyl-2-oxopentanoate as well as recombinant hosts for producing isoprene.

公开(公告)号：US20130183728A1

公开(公告)日：2013-07-18

申请号：US13715981

申请日：2012-12-14

Applicant: INVISTA North America S.a.r.l.

Inventor： Adriana L. Botes ,  Alex Van Eck Conradie

IPC: C12P7/44 ,  C12P17/10 ,  C12P7/18 ,  C12P13/00

CPC classification number: C12N9/0008 ,  C12N15/52 ,  C12P7/18 ,  C12P7/42 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P17/10 ,  C12Y102/02001

Abstract: This document describes biochemical pathways for producing adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine or 1,6-hexanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C6 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on CoA-dependent elongation enzymes or analogues enzymes associated with the carbon storage pathways from polyhydroxyalkanoate accumulating bacteria.

公开(公告)号：US20180057843A1

公开(公告)日：2018-03-01

申请号：US15674978

申请日：2017-08-11

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Adriana Leonora Botes ,  Alex Van Eck Conradie

IPC: C12P5/02

CPC classification number: C12P5/026 ,  Y02E50/343

Abstract: The document provides methods for biosynthesizing isobutene using one or more isolated enzymes such as one or more of an enoyl-CoA dehydratase, a 2-hydroxyacyl-CoA dehydratase, an isovaleryl-CoA/acyl-CoA dehydrogenase and a mevalonate diphosphate decarboxylase, or using recombinant host cells expressing one or more such enzymes.

公开(公告)号：US20160355843A1

公开(公告)日：2016-12-08

申请号：US15211237

申请日：2016-07-15

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Paul S. Pearlman ,  Changlin Chen ,  Adriana Leonora Botes ,  Alex Van Eck Conradie

IPC: C12P5/02 ,  C08J5/00 ,  C07C11/167 ,  C08F136/06 ,  C12N15/52 ,  C12N9/88

CPC classification number: C12P5/026 ,  C07C11/167 ,  C08F136/06 ,  C08J5/00 ,  C08J2347/00 ,  C12N9/001 ,  C12N9/88 ,  C12N15/52 ,  C12P5/02 ,  C12Y103/08 ,  C12Y401/01033 ,  C12Y402/01 ,  C12Y402/03027 ,  Y02E50/343 ,  Y02P20/52

Abstract: This document describes biochemical pathways for producing butadiene by forming two vinyl groups in a butadiene synthesis substrate. These pathways described herein rely on enzymes such as mevalonate diphosphate decarboxylase, isoprene synthase, and dehydratases for the final enzymatic step.