公开(公告)号：US20190338320A1

公开(公告)日：2019-11-07

申请号：US16399155

申请日：2019-04-30

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Alexander Brett FOSTER ,  Cristina Serrano AMATRIAIN ,  Gary J. Smith ,  Paul Sheldon Pearlman ,  Mark Paul Taylor

IPC: C12P7/62

Abstract: Provided herein are methods for generating cellular biomass in continuous aerobic fermentation systems. The biomass yield, and the concentration of polyhydroxyalkanoate within the biomass, are each directed to advantageous levels by operating the continuous fermentation system under particular nutrient limitation conditions. Also provided are biomass produced using the provided methods, and animal feed compositions including the provided biomass.

公开(公告)号：US20190352674A1

公开(公告)日：2019-11-21

申请号：US16399145

申请日：2019-04-30

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Alexander Brett FOSTER ,  Cristina Serrano AMATRIAIN ,  Gary J. Smith ,  Paul Sheldon Pearlman ,  Mark Paul Taylor ,  Jonathan Combe ,  Daniel Bawdon

IPC: C12P7/04 ,  C12N1/20 ,  C12P7/06 ,  C12P7/42 ,  C12N9/10 ,  C12N9/88 ,  C12N9/04

Abstract: Provided herein are methods for increasing the yield of an extracellular product synthesized by an organism cultured in a continuous aerobic fermentation system. The extracellular product yield is increased through the use of an organism modified to decreased production of polyhydroxyalkanoate, to increase production of the extracellular product, and to include promoters that can be inducible in response to nutrient limitation conditions. The extracellular product yield is also increased by operating the continuous fermentation system under particular nutrient limitation conditions. Also provided are non-naturally occurring organisms that have been modified for use with the provided methods, and extracellular products made using the provided methods.

公开(公告)号：US20190316072A1

公开(公告)日：2019-10-17

申请号：US16372106

申请日：2019-04-01

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Gary J. Smith ,  Paul S. Pearlman ,  Gregory S. Kirby

IPC: C12M1/00 ,  C12P7/62 ,  C12M1/34

Abstract: Provided herein are systems and methods for recycling and supplementing off-gas from a gas fed reaction process. The systems and methods are particularly useful for bioprocesses that convert hydrogen gas into one or more biosynthetic products. By maintaining separate hydrogen and oxygen feed gas streams, and forming a recycle gas that introduces a target component of the supply gas to the bioreactor within a target concentration range, the yields, productivities, and safety profiles of the bioprocess can be enhanced.

公开(公告)号：US20190300839A1

公开(公告)日：2019-10-03

申请号：US16372099

申请日：2019-04-01

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Gary J. Smith ,  Paul S. Pearlman ,  Gregory S. Kirby

IPC: C12M1/34

Abstract: The present disclosure provides methods for controlling oxygen concentration during aerobic biosynthesis, e.g., fermentation. The method may comprise feeding an oxygen-containing gas into a vessel including a fermentation feedstock and reacting the fermentation feedstock with the oxygen-containing gas to form a broth including a gaseous phase dispersed within the broth. The gaseous phase may comprise any unreacted oxygen from the oxygen-containing gas. The method further includes reducing the concentration of the unreacted oxygen in the dispersed gaseous phase to less than the limiting oxygen concentration (“LOC”) for flammability before separating the gaseous phase from the fermentation broth. The concentration of the unreacted oxygen in the gaseous phase is reduced by employing oxygen removal schemes or oxygen dilution schemes.

公开(公告)号：US20190300838A1

公开(公告)日：2019-10-03

申请号：US16372092

申请日：2019-04-01

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Gary J. Smith ,  Paul S. Pearlman ,  Gregory S. Kirby

IPC: C12M1/34 ,  C12M1/107 ,  C12M1/00 ,  C12M1/36

Abstract: Disclosed are materials and methods for managing aerobic biosynthesis. The materials include a fermenter system comprising a fermenter, a microorganism provided to the fermenter, and at least two control loops. The methods are directed to measuring and controlling different oxygen concentrations within the fermenter.