公开(公告)号：US20180023104A1

公开(公告)日：2018-01-25

申请号：US15656482

申请日：2017-07-21

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Stephen Cartman ,  Jonathan Combe ,  Alexander B. Foster ,  Jonathan Kennedy

IPC: C12P13/00 ,  C08G69/10 ,  C12N9/10 ,  C12N9/04 ,  C12N9/02 ,  C12N15/52

CPC classification number: C12P13/005 ,  C08G69/08 ,  C08G69/10 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1096 ,  C12N15/52 ,  C12Y101/01021 ,  C12Y102/01003 ,  C12Y102/99006 ,  C12Y203/01009 ,  C12Y206/01

Abstract: This disclosure relates to genome-scale attenuation or knockout strategies for directing carbon flux to certain carbon based building blocks within the 7-aminoheptanoic acid (7-AHA) and 6-aminohexanoic acid (6-AHA) biosynthesis pathways, for example, to achieve reduced flux to unwanted side products while achieving increased production of desired intermediates and end products. This disclosure also relates to non-naturally occurring mutant bacterial strains comprising one or more gene disruptions in aldehyde reductase and/or aldehyde dehydrogenase genes that are generated to direct carbon flux to certain carbon based building blocks. This disclosure further relates to a method for enhancing production of carbon based building blocks by generating non-naturally occurring mutant bacterial strains, culturing said mutant bacterial strains in the presence of suitable substrates or under desired growth conditions, and substantially purifying the desired end product.