公开(公告)号：US20180094282A1

公开(公告)日：2018-04-05

申请号：US15717065

申请日：2017-09-27

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Stephen Thomas Cartman ,  Alexander Brett Foster ,  Ana Teresa dos Santos Brito Mendes ,  Mark Paul Taylor

IPC: C12P5/00 ,  C12R1/01

Abstract: Methods and compositions for synthesizing dienes and derivative thereof, such as isoprene, in Cupriavidus necator are provided.

公开(公告)号：US20190002926A1

公开(公告)日：2019-01-03

申请号：US16022878

申请日：2018-06-29

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Stephen Thomas Cartman ,  Jonathan Paul Combe ,  Leonard Keith Pattenden ,  Andrew Shaw ,  Massimiliano Zampini

IPC: C12P5/00 ,  C12N15/74

Abstract: Genetically engineered hosts and methods for their production and use in synthesizing hydrocarbons are provided.

公开(公告)号：US20190284564A1

公开(公告)日：2019-09-19

申请号：US16337491

申请日：2017-09-27

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Daniel Bawdon ,  Stephen Thomas Cartman ,  Jonathan Kennedy

IPC: C12N15/52 ,  C12N15/74 ,  C12N9/22

Abstract: Nonnaturally occurring host cells altered to increase their ability to transfer genetic molecules into the host cells as compared to an unaltered host cell are provided. Also provided are methods for identifying endogenous loci of a host cell which inhibit transformation efficiency and/or electroporation of genetic molecules into the cell as well as methods for producing nonnaturally occurring host cells with enhanced transformation efficiency and/or the modified ability to allow for genomic integration of an exogenous DNA sequence via electroporation. Methods for producing biochemicals and products produced with the nonnaturally occurring host cells are also provided. In particular, a host Cupriavidus necator cell is provided in which a chromosomal gene encoding a restriction endonuclease has been disrupted, leading to an increase in transformation efficiency.

公开(公告)号：US10801046B2

公开(公告)日：2020-10-13

申请号：US15659505

申请日：2017-07-25

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Alexander Brett Foster ,  Stephen Thomas Cartman ,  Jonathan Kennedy ,  William Joseph Simmons

IPC: C12P7/62 ,  C12P7/42 ,  C12N15/09 ,  C12P7/64 ,  C12N15/52 ,  C12P13/00 ,  C12P7/24 ,  C12P7/40 ,  C07C47/12 ,  C07C47/19 ,  C07C55/16 ,  C07C59/147 ,  C07C223/02 ,  C07C225/06 ,  C07C229/08 ,  C12N9/04 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88 ,  C12P7/18 ,  C12P7/44

Abstract: This document describes biochemical pathways for producing a difunctional product having an odd number of carbon atoms in vitro or in a recombinant host, or salts or derivatives thereof, by forming two terminal functional groups selected from carboxyl, amine, formyl, and hydroxyl groups in an aliphatic carbon chain backbone having an odd number of carbon atoms synthesized from (i) acetyl-CoA and propanedioyl-CoA via one or more cycles of methyl ester shielded carbon chain elongation or (ii) propanedioyl-[acp] via one or more cycles of methyl ester shielded carbon chain elongation. The biochemical pathways and metabolic engineering and cultivation strategies described herein rely on enzymes or homologs accepting methyl ester shielded aliphatic carbon chain backbones and maintaining the methyl ester shield for at least one further enzymatic step following one or more cycles of methyl ester shielded carbon chain elongation.

公开(公告)号：US10538788B2

公开(公告)日：2020-01-21

申请号：US15717065

申请日：2017-09-27

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Stephen Thomas Cartman ,  Alexander Brett Foster ,  Ana Teresa dos Santos Brito Mendes Roberts ,  Mark Paul Taylor

IPC: C12N9/88 ,  C12P5/00 ,  C12R1/01 ,  C07C11/12 ,  C08F136/08 ,  C12N9/90 ,  C12N15/74

Abstract: Methods and compositions for synthesizing dienes and derivative thereof, such as isoprene, in Cupriavidus necator are provided.

公开(公告)号：US20190233853A1

公开(公告)日：2019-08-01

申请号：US16264765

申请日：2019-02-01

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Cristina Serrano Amatriain ,  Alexander Brett Foster ,  Stephen Thomas Cartman

IPC: C12P7/52 ,  C12N9/88 ,  C12N9/02 ,  C12N9/16

CPC classification number: C12P7/52 ,  C12N9/0008 ,  C12N9/16 ,  C12N9/88 ,  C12Y102/01003 ,  C12Y301/03021 ,  C12Y402/0103

Abstract: Methods and materials for the production of beta hydroxy acids, such as 3-hydroxypropanoic acid (3-HP) and/or derivatives thereof and/or compounds related thereto, are provided. Also provided are products produced in accordance with these methods and materials.

公开(公告)号：US20190218577A1

公开(公告)日：2019-07-18

申请号：US16337481

申请日：2017-09-27

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Stephen Thomas Cartman ,  Alexander Brett Foster ,  Ana Teresa De Santos Brito Mendes Roberts ,  Mark Paul Taylor

IPC: C12P5/00 ,  C12N9/90 ,  C12N9/88 ,  C07C11/12 ,  C08F136/08

CPC classification number: C12P5/007 ,  C07C11/12 ,  C08F136/08 ,  C12N9/88 ,  C12N9/90 ,  C12N15/74 ,  C12N2330/30 ,  C12P5/026 ,  C12R1/01 ,  C12Y402/03027 ,  C12Y503/03002 ,  Y02E50/343

Abstract: Methods and compositions for synthesizing dienes and derivative thereof, such as isoprene, in Cupriavidus necator are provided.

公开(公告)号：US20180100160A1

公开(公告)日：2018-04-12

申请号：US15717216

申请日：2017-09-27

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Daniel Bawdon ,  Stephen Thomas Cartman ,  Jonathan Kennedy

IPC: C12N15/74 ,  C12N15/64 ,  C12N13/00

CPC classification number: C12N15/74 ,  C12N9/22 ,  C12N13/00 ,  C12N15/64

Abstract: Nonnaturally occurring host cells altered to increase their ability to transfer genetic molecules into the host cells as compared to an unaltered host cell are provided. Also provided are methods for identifying endogenous loci of a host cell which inhibit transformation efficiency and/or electroporation of genetic molecules into the cell as well as methods for producing nonnaturally occurring host cells with enhanced transformation efficiency and/or the modified ability to allow for genomic integration of an exogenous DNA sequence via electroporation. Methods for producing biochemicals and products produced with the nonnaturally occurring host cells are also provided.