公开(公告)号：WO2014105788A8

公开(公告)日：2015-05-28

申请号：PCT/US2013077411

申请日：2013-12-23

Applicant: INVISTA TECH SARL

Inventor： BOTES ADRIANA LEONORA ,  CONRADIE ALEX VAN ECK ,  CHEN CHANGLIN ,  PEARLMAN PAUL S

IPC: C12P7/18 ,  C12N15/63 ,  C12P7/42 ,  C12P7/44 ,  C12P13/00

CPC classification number: C12P13/001 ,  C12N9/0008 ,  C12N9/1096 ,  C12N9/16 ,  C12P7/18 ,  C12P7/42 ,  C12P7/44 ,  C12P13/002 ,  C12Y102/99006 ,  C12Y301/02002

Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the carbon chain elongation enzymes or homologs thereof associated with the cyclohexane carboxylate biosynthesis from Syntrophus aciditrophicus or 2-aminoadipate lysine biosynthesis.

Abstract translation: 该文献描述了通过在C7脂肪族主链基质中形成由羧基，胺或羟基组成的两个末端官能团来生产庚二酸，7-氨基庚酸，7-羟基庚酸，七亚甲基二胺或1,7-庚二醇的生化途径。 本文描述的这些途径，代谢工程和培养策略依赖于与酸性营养不良食物或2-氨基己二酸赖氨酸生物合成的环己烷羧酸盐生物合成相关的碳链延长酶或其同系物。

公开(公告)号：WO2013090837A3

公开(公告)日：2014-03-13

申请号：PCT/US2012069934

申请日：2012-12-14

Applicant: INVISTA TECH SARL

Inventor： BOTES ADRIANA ,  CONRADIE ALEX VAN ECK

IPC: C12P7/18 ,  C12P7/44 ,  C12P13/00 ,  C12P17/10

CPC classification number: C12P7/44 ,  C12N15/52 ,  C12P7/18 ,  C12P13/001 ,  C12P13/005 ,  C12P17/10

Abstract: This document describes biochemical pathways for producing adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine or 1,6-hexanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C6 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on CoA-dependent elongation enzymes or analogues enzymes associated with the carbon storage pathways from polyhydroxyalkanoate accumulating bacteria.

Abstract translation: 该文献描述了通过在C6脂肪族主链基质中形成由羧基，胺或羟基组成的两个末端官能团来生产己二酸，己内酰胺，6-氨基己酸，六亚甲基二胺或1,6-己二醇的生化途径。 本文描述的这些途径，代谢工程和培养策略依赖于CoA依赖性延长酶或类似物与聚羟基链烷酸酯累积细菌的碳储存途径相关的酶。

公开(公告)号：WO2016175901A3

公开(公告)日：2016-12-08

申请号：PCT/US2015067099

申请日：2015-12-21

Applicant: INVISTA NORTH AMERICA S Á R L ,  INVISTA TECH SARL

Inventor： BOTES ADRIANA LEONORA ,  CONRADIE ALEX VAN ECK

IPC: C12P7/26 ,  C07H19/207 ,  C12P7/42 ,  C12P7/62

CPC classification number: C12P19/32 ,  C07H1/00 ,  C07H19/207 ,  C12N9/1029 ,  C12P7/24 ,  C12P7/26 ,  C12P7/42 ,  C12P13/001 ,  C12P13/005 ,  C12Y203/01

Abstract: This document describes materials and methods for, for example, producing 6-hydroxyhexanoic acid using a β-ketothiolase or synthase and an alcohol O-acetyltransferase to form a 6-acetyloxy-3-oxohexanoyl-CoA intermediate. This document describes biochemical pathways for producing 6-hydroxyhexanoic acid using a β-ketothiolase or synthase and an alcohol O-acetyltransferase to form a 6-acetyloxy-3-oxohexanoyl-CoA intermediate. 6-hydroxyhexanoic acid can be enzymatically converted to adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine or 1,6-hexanediol. This document also describes recombinant hosts producing 6-hydroxyhexanoic acid as well as adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine and 1,6-hexanediol.

Abstract translation: 该文献描述了例如使用β-酮硫解酶或合酶和醇O-乙酰转移酶产生6-羟基己酸以形成6-乙酰氧基-3-氧代己酰-CoA中间体的材料和方法。 本文件描述了使用β-酮硫解酶或合酶和醇O-乙酰转移酶生成6-羟基己酸的生物化学途径以形成6-乙酰氧基-3-氧代己酰-CoA中间体。 6-羟基己酸可以酶促转化为己二酸，己内酰胺，6-氨基己酸，六亚甲基二胺或1,6-己二醇。 该文献还描述了生产6-羟基己酸以及己二酸，己内酰胺，6-氨基己酸，六亚甲基二胺和1,6-己二醇的重组宿主。

公开(公告)号：WO2013188546A3

公开(公告)日：2014-03-27

申请号：PCT/US2013045430

申请日：2013-06-12

Applicant: INVISTA TECH SARL

Inventor： PEARLMAN PAUL S ,  CHEN CHANGLIN ,  BOTES ADRIANA ,  CONRADIE ALEX VAN ECK

IPC: C12P5/02 ,  C07C11/167 ,  C12N9/00 ,  C12N9/02

CPC classification number: C07C11/167 ,  C12N9/0004 ,  C12P5/026 ,  Y02E50/343

Abstract: This document describes biochemical pathways for producing butadiene by forming two vinyl groups in a butadiene synthesis substrate. These pathways described herein rely on enzymes such as mevalonate diphosphate decarboxylase, isoprene synthase, and dehydratases for the final enzymatic step.

Abstract translation: 该文献描述了通过在丁二烯合成底物中形成两个乙烯基来生产丁二烯的生化途径。 本文描述的这些途径依赖于酶，例如甲羟戊酸二磷酸脱羧酶，异戊二烯合酶和用于最终酶促步骤的脱水酶。

公开(公告)号：WO2015195710A8

公开(公告)日：2016-12-08

申请号：PCT/US2015036095

申请日：2015-06-16

Applicant: INVISTA TECH SARL

Inventor： BOTES ADRIANA LEONORA ,  CONRADIE ALEX VAN ECK

IPC: C12P7/44 ,  C12N9/10 ,  C12N9/16 ,  C12N9/18 ,  C12N15/52 ,  C12P7/62

CPC classification number: C12P7/62 ,  C07C69/42 ,  C07C229/08 ,  C12N9/0008 ,  C12N9/1007 ,  C12N9/1096 ,  C12N9/16 ,  C12N9/18 ,  C12N15/52 ,  C12P7/42 ,  C12P7/44 ,  C12P13/001 ,  C12Y102/99006 ,  C12Y201/01197 ,  C12Y206/01018 ,  C12Y206/01038 ,  C12Y301/01085

Abstract: This document describes biochemical pathways for producing 2,4-pentadienoyl-CoA by forming one or two terminal functional groups, comprised of carboxyl or hydroxyl group, in a C5 backbone substrate such as glutaryl-CoA, glutaryl-[acp] or glutarate methyl ester. 2,4-pentadienoyl-CoA can be enzymatically converted to 1,3-butadiene.

Abstract translation: 本文件描述了通过在C5主链基质如戊二酰辅酶A，戊二酰 - [acp]或戊二酸甲酯中形成一个或两个由羧基或羟基组成的末端官能团来生产2,4-戊二烯酰辅酶A的生化途径 。 2,4-戊二烯酰辅酶A可以酶促转化为1,3-丁二烯。

公开(公告)号：WO2013096898A3

公开(公告)日：2014-03-13

申请号：PCT/US2012071472

申请日：2012-12-21

Applicant: INVISTA TECH SARL

Inventor： PEARLMAN PAUL S ,  CHEN CHANGLIN ,  BOTES ADRIANA ,  CONRADIE ALEX VAN ECK ,  HERZOG BENJAMIN D

IPC: C12P7/44 ,  C08L77/02 ,  C12P7/42 ,  C12P13/00 ,  C12P13/02 ,  C12P17/10

CPC classification number: C12P13/02 ,  C08G69/08 ,  C08G69/26 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P7/50 ,  C12P13/001 ,  C12P13/005 ,  C12P13/04 ,  C12P17/10

Abstract: Embodiments of the present invention relate to methods for the biosynthesis of di- or trifunctional C7 alkanes in the presence of isolated enzymes or in the presence of a recombinant host cell expressing those enzymes. The di- or trifunctional C7 alkanes are useful as intermediates in the production of nylon-7, nylon-7,x, nylon-x,7, and polyesters.

Abstract translation: 本发明的实施方案涉及在分离的酶存在下或在表达这些酶的重组宿主细胞存在下生物合成二官能或三官能C7烷烃的方法。 二 - 或三官能C7烷烃可用作生产尼龙-7，尼龙-7，x，尼龙-X，7和聚酯的中间体。