公开(公告)号：US20160168610A1

公开(公告)日：2016-06-16

申请号：US14908659

申请日：2014-07-29

Applicant: INVISTA TECHNOLOGIES S.a.r.l.

Inventor： Alex Van Eck Conradie ,  Adriana Leonora Botes

IPC: C12P19/32

CPC classification number: C12P19/32 ,  C12N9/1029 ,  C12P7/26 ,  C12P7/40 ,  C12Y203/01016

Abstract: This document describes biochemical pathways that include the production of 3-oxopent-4-enoyl-CoA by condensation of acryloyl-CoA and acetyl-CoA using a β-ketothiolase with a SER-HIS-HIS catalytic triad. These pathways described herein rely on enzymes such as, inter alia, dehydrogenases, dehydratases and β-ketothiolases.

Abstract translation: 本文件描述了生物化学途径，其包括通过使用α-氧化还原酶与SER-HIS-HIS催化三单元组缩合丙烯酰辅酶A和乙酰辅酶A来生产3-氧代-4-烯酰辅酶A。 本文描述的这些途径依赖于酶，例如脱氢酶，脱水酶和β-酮硫解酶。

公开(公告)号：US20160201094A1

公开(公告)日：2016-07-14

申请号：US14914113

申请日：2014-08-28

Applicant: INVISTA TECHNOLOGIES S.A.R.L.

Inventor： Adriana Leonora Botes ,  Alex Van Eck Conradie

IPC: C12P7/42 ,  C12N9/02

CPC classification number: C12P7/40 ,  C08F20/10 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/13 ,  C12N9/16 ,  C12N15/52 ,  C12P7/42 ,  C12Y102/01039 ,  C12Y103/99 ,  C12Y208/03 ,  C12Y208/03001 ,  C12Y301/02

Abstract: This document describes biochemical pathways for producing methacrylate from precursors such as pyruvate via isobutyraldehyde and isobutyryl-CoA, using enzymes such as one or more thioesterases, transferases, or dehydrogenases, as well as recombinant hosts expressing one or more of such enzymes.

公开(公告)号：US20150337275A1

公开(公告)日：2015-11-26

申请号：US14367484

申请日：2012-12-21

Applicant: INVISTA TECHNOLOGIES S.a.r.l.

Inventor： Paul S. Pearlman ,  Changlin Chen ,  Adriana L. Botes ,  Alex Van Eck Conradie ,  Benjamin D. Herzog

IPC: C12N9/10 ,  C12P7/40 ,  C12P13/00 ,  C12P13/04 ,  C12P13/02 ,  C12N9/16 ,  C12N9/02 ,  C12N9/00 ,  C12P7/44 ,  C12P17/10

Abstract: Embodiments of the present invention relate to methods for the biosynthesis of di- or trifunctional C7 alkanes in the presence of isolated enzymes or in the presence of a recombinant host cell expressing those enzymes. The di- or trifunctional C7 alkanes are useful as intermediates in the production of nylon-7, nylon-7,x, nylon-x,7, and polyesters.

Abstract translation: 本发明的实施方案涉及在分离的酶存在下或在表达这些酶的重组宿主细胞的存在下生物合成二官能或三官能C7烷烃的方法。 二 - 或三官能C7烷烃可用作生产尼龙-7，尼龙-7，x，尼龙-X，7和聚酯的中间体。

公开(公告)号：US20160237461A1

公开(公告)日：2016-08-18

申请号：US15051989

申请日：2016-02-24

Applicant: INVISTA TECHNOLOGIES S.A.R.L.

Inventor： Adriana Leonora Botes ,  Alex Van Eck Conradie

IPC: C12P7/40 ,  C08F20/10 ,  C12N15/52 ,  C12N9/02 ,  C12N9/10

CPC classification number: C12P7/40 ,  C08F20/10 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/13 ,  C12N9/16 ,  C12N15/52 ,  C12P7/42 ,  C12Y102/01039 ,  C12Y103/99 ,  C12Y208/03 ,  C12Y208/03001 ,  C12Y301/02

Abstract: This document describes biochemical pathways for producing methacrylate from precursors such as pyruvate via isobutyraldehyde and isobutyryl-CoA, using enzymes such as one or more thioesterases, transferases, or dehydrogenases, as well as recombinant hosts expressing one or more of such enzymes.

Abstract translation: 该文献描述了使用诸如一种或多种硫酯酶，转移酶或脱氢酶的酶以及表达一种或多种这样的酶的重组宿主从异构丁醛和异丁酰辅酶A等前体生产甲基丙烯酸酯的生化途径。