公开(公告)号：US20190154682A1

公开(公告)日：2019-05-23

申请号：US16260413

申请日：2019-01-29

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Hiroyuki NOJI ,  Ryota IINO ,  Suguru ARAKI

IPC: G01N33/543 ,  G01N21/64 ,  B01L3/00

CPC classification number: G01N33/54386 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/50853 ,  B01L2200/0642 ,  B01L2200/0668 ,  B01L2200/12 ,  B01L2300/0627 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0858 ,  B01L2300/0877 ,  B01L2300/161 ,  B01L2300/165 ,  G01N21/6452 ,  G01N33/54306 ,  G01N33/54313

Abstract: This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41′) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41′) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).

公开(公告)号：US20170115284A1

公开(公告)日：2017-04-27

申请号：US15351522

申请日：2016-11-15

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Hiroyuki NOJI ,  Ryota IINO ,  Suguru ARAKI

IPC: G01N33/543 ,  G01N21/64 ,  B01L3/00

CPC classification number: G01N33/54386 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/50853 ,  B01L2200/0642 ,  B01L2200/0668 ,  B01L2200/12 ,  B01L2300/0627 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0858 ,  B01L2300/0877 ,  B01L2300/161 ,  B01L2300/165 ,  G01N21/6452 ,  G01N33/54306 ,  G01N33/54313

Abstract: This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41′) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41′) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).

公开(公告)号：US20160223531A1

公开(公告)日：2016-08-04

申请号：US15082195

申请日：2016-03-28

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Hiroyuki NOJI ,  Ryota IINO ,  Suguru ARAKI

IPC: G01N33/543 ,  B01L3/00

CPC classification number: G01N33/54386 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/50853 ,  B01L2200/0642 ,  B01L2200/0668 ,  B01L2200/12 ,  B01L2300/0627 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0858 ,  B01L2300/0877 ,  B01L2300/161 ,  B01L2300/165 ,  G01N21/6452 ,  G01N33/54306 ,  G01N33/54313

Abstract: This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41′) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41′) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).

Abstract translation: 本发明提供能够以高灵敏度检测低浓度的目标分子的技术。 本发明包括（i）将含有珠（40），（41'）的亲水性溶剂（42）引入到（a）包括多个容器（13）的下层部分（10）之间的空间（30） ），其每一个仅能够容纳一个珠（41），（41'），并且通过具有疏水性上表面的侧壁（12）和（b）上层部分（20'）彼此分离 ）面向设置有多个插座（13）的表面的下层部分（10）的表面; 和（ii）将疏水性溶剂（43）引入空间（30）的步骤，步骤（ii）在步骤（i）之后进行。