公开(公告)号：US11352652B2

公开(公告)日：2022-06-07

申请号：US16976222

申请日：2019-03-04

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Naoki Takaya ,  Shunsuke Masuo ,  Yukie Kawasaki ,  Hajime Minakawa

IPC: C12P13/00

Abstract: Provided is a novel method for producing 4-aminocinnamic acid from 4-nitrophenylalanine. This method comprises: converting 4-nitrophenylalanine into 4-nitrocinnamic acid; and converting 4-nitrocinnamic acid into 4-aminocinnamic acid.

公开(公告)号：US10053716B2

公开(公告)日：2018-08-21

申请号：US15229661

申请日：2016-08-05

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Kazunobu Konishi ,  Naoki Takaya ,  Shunsuke Masuo ,  Shengmin Zhou

IPC: C12P13/00 ,  C07C229/44 ,  C12N9/88 ,  C12N15/70

CPC classification number: C12P13/005 ,  C07C229/44 ,  C12N9/88 ,  C12N15/70 ,  C12Y403/01024

Abstract: This 4-amino cinnamic acid production method using the enzyme ammonia lyase can efficiently convert 4-amino phenylalanine into 4-amino cinnamic acid. This 4-amino cinnamic acid production method is characterized by converting 4-amino phenylalanine into 4-amino cinnamic acid by using phenylalanine ammonia-lyase, which comprises the amino acid sequence represented in sequence number 2 derived from the Rhodotorula glutinis yeast.

公开(公告)号：US09920343B2

公开(公告)日：2018-03-20

申请号：US15265123

申请日：2016-09-14

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Kazunobu Konishi ,  Naoki Takaya ,  Shunsuke Masuo

IPC: C12P13/22 ,  C12P13/00

CPC classification number: C12P13/222 ,  C12P13/001 ,  C12P13/005 ,  C12P13/22

Abstract: Provided is a method for producing an aniline derivative by fermentation from a carbon source such as glucose. The method comprises the following steps: production of microorganisms capable of producing 1.8 g/L or more of 4-aminophenylalanine (4APhe) under prescribed culture conditions by introducing at least three exogenous genes into microorganisms having the ability to biosynthesize 4-aminophenylpyruvic acid from chorismic acid; and production of at least one aniline derivative selected from the group consisting of 4-aminophenylalanine (4APhe), 4-aminocinnamic acid (4ACA), 2-(4-aminophenyl)aldehyde, 4-aminophenylacetic acid, and 4-aminophenethylethanol (4APE) by bringing these microorganisms into contact with a carbon source under conditions suited to the growth and/or maintenance of these microorganisms.