公开(公告)号：US20220204569A1

公开(公告)日：2022-06-30

申请号：US17601174

申请日：2020-04-09

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Yasushi OKADA ,  Daisuke INO ,  Kazuho IKEDA

IPC: C07K14/195 ,  C12N15/62 ,  C12N15/85 ,  C12N15/88 ,  C12Q1/6876 ,  C07K19/00 ,  A01K67/027 ,  A01K67/033 ,  G01N21/64

Abstract: The present invention provides a novel modified protein which is to be used, as a novel detection tool relating to gene expression, for detecting a chromatin open structure more easily at a higher sensitivity than by the conventional technique. The present invention relates to: a nucleic acid binding fluorescent protein, said protein containing a DNA binding domain in which 3 or more TAL-repeats are repeatedly connected, characterized by binding independently from base sequences; and a method for fluorescent labeling of an open chromatin in a vital cell, said method comprising a step for transferring a gene encoding a nucleic acid binding protein into the vital cell, characterized in that the nucleic acid binding protein is a protein comprising a DNA binding domain, in which 3 or more TAL-repeats are repeatedly connected, and a fluorescent protein directly or indirectly bound thereto and the DNA binding domain binds to a nucleic acid independently from base sequences.

公开(公告)号：US20220177948A1

公开(公告)日：2022-06-09

申请号：US17299018

申请日：2019-12-03

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Yasushi OKADA ,  Tetsuro ARIYOSHI

IPC: C12Q1/6806 ,  C12Q1/6825 ,  C12Q1/6876

Abstract: The present invention provides a fluorogenic RNA capable of visualizing mRNA in cells, particularly, living mammalian cells, and a target RNA fluorescent labeling method using the fluorogenic RNA. The present invention provides a fluorogenic nucleic acid molecule containing a base sequence having two or more fluorescent molecule-binding regions linked via a linker sequence, in which one or more fluorescent molecule-binding aptamer sequences are inserted into a scaffold sequence of each of the fluorescent molecule-binding regions.