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公开(公告)号：CA3155651A1
公开(公告)日：2021-04-01
申请号：CA3155651
申请日：2020-09-25
Applicant: JUMPCODE GENOMICS INC
Inventor： BROWN KEITH
IPC: C12Q1/6869 , C12N15/09 , C12N15/10 , C12N15/113
Abstract: Disclosed herein are methods, compositions, and systems that utilize the consensus sequencing of rolling circle amplified short templates. Methods of determining a nucleic acid sequence can include one or more steps of contacting a nucleic acid in a sample to an endonuclease to cleave a target nucleic acid; ligating the target nucleic acid sequence to form a circular target nucleic acid; hybridizing at least one primer to the circular target molecule to form amplified nucleic acid through rolling circle amplification; and performing sequence analysis of the amplified nucleic acid.
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公开(公告)号：CA3013932A1
公开(公告)日：2017-08-17
申请号：CA3013932
申请日：2017-02-10
Applicant: JUMPCODE GENOMICS INC
Inventor： BROWN KEITH
Abstract: Disclosed herein are methods of long range target specific amplification and sequencing using an RNA intermediate synthesized directly from the target to eliminate clonal amplification of early synthesis errors. Approaches allow for the identification of target-adjacent sequence, such as sequence adjacent to a repeat element target. Also disclosed herein are compositions and kits for amplification and sequencing.
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公开(公告)号：ES2894048T3
公开(公告)日：2022-02-11
申请号：ES15754498
申请日：2015-02-27
Applicant: JUMPCODE GENOMICS INC
Inventor： BROWN KEITH
IPC: A61K38/45 , A61P43/00 , C12Q1/6809 , C12Q1/6869 , C12Q1/6886 , G16H15/00
Abstract: Un procedimiento de monitoreo de inserción de elementos móviles (IEM) que comprende las etapas de (a) obtener información de la secuencia del genoma por secuenciación del ADN de una muestra biológica que comprende un ácido nucleico tumoral de un individuo en un primer momento, donde la información de la secuencia del genoma comprende una pluralidad de bordes de inserción de IEM; (b) revisar la pluralidad de bordes de inserción de IEM para identificar al menos un borde de IEM adyacente a un oncogén; y (c) monitorear una abundancia cuantitativa del al menos un borde de IEM adyacente al oncogén a lo largo del tiempo, donde (d) el monitoreo de la abundancia cuantitativa del al menos un borde de IEM adyacente al oncogén en el transcurso del tiempo comprende obtener una primera muestra de sangre en el primer momento, determinar la abundancia cuantitativa del al menos un borde de IEM en la primera muestra de sangre en el primer momento, obtener una segunda muestra de sangre en un segundo momento, y determinar la abundancia cuantitativa del al menos un borde de IEM en la segunda muestra de sangre en el segundo momento, o donde (e) dicho monitoreo de la abundancia cuantitativa del al menos un borde de IEM adyacente al oncogén en el transcurso del tiempo comprende emplear una muestra de ácido nucleico de una primera muestra de tejido obtenida en un primer momento, determinar la abundancia cuantitativa del al menos un borde de IEM en la primera muestra de ácido nucleico en el primer momento, emplear una segunda muestra de ácido nucleico de una segunda muestra de tejido obtenida en un segundo momento, y determinar la abundancia cuantitativa del al menos un borde de IEM en la segunda muestra de ácido nucleico en el segundo momento.
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公开(公告)号：DK3110975T3
公开(公告)日：2021-10-11
申请号：DK15754498
申请日：2015-02-27
Applicant: JUMPCODE GENOMICS INC
Inventor： BROWN KEITH
IPC: A61K38/45 , A61P43/00 , C12Q1/6809 , C12Q1/6869 , C12Q1/6886 , G16H15/00
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公开(公告)号：AU2015222802B2
公开(公告)日：2021-07-01
申请号：AU2015222802
申请日：2015-02-27
Applicant: JUMPCODE GENOMICS INC
Inventor： BROWN KEITH
IPC: C12Q1/68
Abstract: Methods and compositions related to the use of Mobile Element Insertions and their adjacent genomic sequences. Methods using MEIs as markers for cellular proliferation, as targets for pharmaceuticals, as markers for tissue fingerprinting and in related methods and compositions are disclosed herein. Methods and compositions relate to the detection, treatment and ongoing monitoring of cell proliferation events, cancer, and deleterious effects of mobile elements in aging, and to the selection, use and monitoring of the success of treatment regimens to address these conditions.
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Patent Agency Ranking
公开(公告)号：AU2021209101A1
公开(公告)日：2022-08-18
申请号：AU2021209101
申请日：2021-01-15
Applicant: JUMPCODE GENOMICS INC
Inventor： BROWN KEITH
IPC: C12Q1/6806 , C12Q1/6853 , C12Q1/6876
Abstract: Provided herein are methods and compositions for creating a sequencing library comprising a target nucleic acid. Methods herein can comprise: contacting a nucleic acid sample to a first population of primers, a polymerase, dNTPs, and labeled ddNTPs; performing an extension reaction thereby creating an labeled extension product; contacting the extension product to a second population of primers to create a double stranded extension product comprising the target nucleic acid; contacting the double stranded extension product to a target specific enzyme under conditions allowing cleavage of at least a subset of the double stranded extension product thereby creating a cleaved target nucleic acid; and isolating the cleaved target nucleic acid.
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公开(公告)号：AU2021240134A1
公开(公告)日：2021-10-28
申请号：AU2021240134
申请日：2021-09-28
Applicant: JUMPCODE GENOMICS INC
Inventor： BROWN KEITH
IPC: C12Q1/68
Abstract: Methods and compositions related to the use of Mobile Element Insertions and their adjacent genomic sequences. Methods using MEIs as markers for cellular proliferation, as targets for pharmaceuticals, as markers for tissue fingerprinting and in related methods and compositions are disclosed herein. Methods and compositions relate to the detection, treatment and ongoing monitoring of cell proliferation events, cancer, and deleterious effects of mobile elements in aging, and to the selection, use and monitoring of the success of treatment regimens to address these conditions.
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公开(公告)号：AU2017217868A1
公开(公告)日：2018-08-23
申请号：AU2017217868
申请日：2017-02-10
Applicant: JUMPCODE GENOMICS INC
Inventor： BROWN KEITH
Abstract: Disclosed herein are methods of long range target specific amplification and sequencing using an RNA intermediate synthesized directly from the target to eliminate clonal amplification of early synthesis errors. Approaches allow for the identification of target-adjacent sequence, such as sequence adjacent to a repeat element target. Also disclosed herein are compositions and kits for amplification and sequencing.
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公开(公告)号：AU2020372488A1
公开(公告)日：2022-05-19
申请号：AU2020372488
申请日：2020-10-23
Applicant: JUMPCODE GENOMICS INC
Inventor： BROWN KEITH
Abstract: Provided are methods for preparing samples for sequencing. Also provided are methods for sequence analysis. Also provided are methods for classifying multiple aspects of a nucleotide repeat expansion disorder in a single sequencing assay. Also provided are methods for genotyping a target nucleic acid sequence.
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公开(公告)号：AU2020354648A1
公开(公告)日：2022-04-14
申请号：AU2020354648
申请日：2020-09-25
Applicant: JUMPCODE GENOMICS INC
Inventor： BROWN KEITH
IPC: C12Q1/6869 , C12N15/09 , C12N15/10 , C12N15/113
Abstract: Disclosed herein are methods, compositions, and systems that utilize the consensus sequencing of rolling circle amplified short templates. Methods of determining a nucleic acid sequence can include one or more steps of contacting a nucleic acid in a sample to an endonuclease to cleave a target nucleic acid; ligating the target nucleic acid sequence to form a circular target nucleic acid; hybridizing at least one primer to the circular target molecule to form amplified nucleic acid through rolling circle amplification; and performing sequence analysis of the amplified nucleic acid.
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