公开(公告)号：EP1548481A1

公开(公告)日：2005-06-29

申请号：EP03808886.0

申请日：2003-09-18

Applicant: Japan Science and Technology Agency

Inventor： HAYASHI, Terutake ,  MAEKAWA, Katsuhiro ,  SHIBATA, Takayuki

IPC: G02B21/00 ,  G02B21/06 ,  G01N37/00 ,  C12N15/09

CPC classification number: G01N21/6458 ,  G01N21/21 ,  G01N21/6445 ,  G01N21/6452 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2201/0675 ,  G02B21/0024 ,  G02B21/0068 ,  G02B21/0076

Abstract: The present invention relates to a confocal microscope and the measuring methods of fluorescence and the polarized light using the same, and said confocal microscope is provided with the inlet optical part (10, 10') to let the polarized light from an illuminating light source (11) onto an object to be observed (2) via a matrix type liquid crystal device (22) provided with a microlens array (21) on its top part, and an objective lens (23), the light detecting part (30, 30') to detect the reflected or the fluorescent light from the object to be observed, and the liquid crystal control subpart (52) to control a liquid crystal device (22), and it transmits the light passing through said microlens array (21) from each microlens to each pixel (22a) of the liquid crystal device (22), and makes a plurality of foci (24) on the object to be observed (2) by the objective lens (23), as well as controls polarization directions of the lights transmitted through each pixel of the liquid crystal device (22) using the liquid crystal control subpart (52) so that they are made mutually orthogonal.

Abstract translation: 本发明涉及共焦显微镜以及使用其的荧光和偏振光的测量方法，并且所述共聚焦显微镜设置有入射光学部件（10,10'），以使来自照明光源的偏振光 11）通过在其顶部上设置有微透镜阵列（21）的矩阵型液晶装置（22）和物镜（23）到待观察的物体（2）上，所述光检测部分（30,30） '）来检测来自被观察物体的反射光或荧光，以及液晶控制部（52）来控制液晶装置（22），将透过所述微透镜阵列（21）的光透过 对液晶装置（22）的每个像素（22a）进行微透镜，并且通过物镜（23）在被观察物体（2）上形成多个焦点（24），并且控制偏振方向 光通过液晶装置的每个像素传输 e（22）使用液晶控制子部（52），使得它们相互正交。