公开(公告)号：US10098841B2

公开(公告)日：2018-10-16

申请号：US13870002

申请日：2013-04-25

Applicant: Japan Science and Technology Agency

Inventor： Munetaka Kunishima

IPC: A61K9/127 ,  B01J13/02 ,  B01J13/12

Abstract: Provided is a bilayer membrane vesicle capable of undergoing a phase transition. The bilayer membrane vesicle includes: (a) a fatty acid salt having 6 to 20 carbon atoms; (b) an alcohol or an amine compound having an aliphatic chain of 6 to 20 carbon atoms; and (c) an artificial synthetic lipid or a phospholipid capable of forming a bilayer membrane. Preferably, this bilayer membrane vesicle further contains (d) a tertiary amine as a component of the membrane. Also provided is a method of inducing a phase transition of a bilayer membrane vesicle, the method including the step of adding a dehydrating condensing agent or a dehydrating condensing agent precursor having the property of accumulating at an interface to the bilayer membrane vesicle. By causing the lipids that form a molecular aggregate to chemically change, it is possible to change the physical property and the morphology of the molecular aggregate and control the timing of phase transitions such as membrane fusion. In the membrane fusion, for example, fusion can occur without leakage of the contents of the bilayer membrane vesicle.

公开(公告)号：US20130251788A1

公开(公告)日：2013-09-26

申请号：US13870002

申请日：2013-04-25

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Munetaka Kunishima

IPC: A61K9/127

CPC classification number: A61K9/1277 ,  A61K9/127 ,  B01J13/02 ,  B01J13/12 ,  Y10T428/1352 ,  Y10T428/2984 ,  Y10T428/31761

Abstract: Provided is a bilayer membrane vesicle capable of undergoing a phase transition. The bilayer membrane vesicle includes: (a) a fatty acid salt having 6 to 20 carbon atoms; (b) an alcohol or an amine compound having an aliphatic chain of 6 to 20 carbon atoms; and (c) an artificial synthetic lipid or a phospholipid capable of forming a bilayer membrane. Preferably, this bilayer membrane vesicle further contains (d) a tertiary amine as a component of the membrane. Also provided is a method of inducing a phase transition of a bilayer membrane vesicle, the method including the step of adding a dehydrating condensing agent or a dehydrating condensing agent precursor having the property of accumulating at an interface to the bilayer membrane vesicle. By causing the lipids that form a molecular aggregate to chemically change, it is possible to change the physical property and the morphology of the molecular aggregate and control the timing of phase transitions such as membrane fusion. In the membrane fusion, for example, fusion can occur without leakage of the contents of the bilayer membrane vesicle.

Abstract translation: 提供能够进行相变的双层膜囊泡。 双层膜泡囊包括：（a）具有6至20个碳原子的脂肪酸盐; （b）具有6至20个碳原子的脂族链的醇或胺化合物; 和（c）能够形成双层膜的人造合成脂质或磷脂。 优选地，该双层膜泡囊还含有（d）作为膜的组分的叔胺。 还提供了诱导双层膜囊泡相变的方法，该方法包括以下步骤：将具有在界面处积累的性质的脱水缩合剂或脱水缩合剂前体添加到双层膜泡囊。 通过使形成分子聚集体的脂质发生化学变化，可以改变分子聚集体的物理性质和形态，并且控制相变如时间的融合。 例如，在膜融合中，可以发生融合，而不会泄漏双层膜囊泡的内容物。