公开(公告)号：US20220025351A1

公开(公告)日：2022-01-27

申请号：US17276222

申请日：2019-09-03

Applicant: KANEKA CORPORATION

Inventor： Mirai TANAKA ,  Hiroyuki KANAMARU ,  Noriyuki ITO ,  Hiroaki YASUKOUCHI

IPC: C12N11/12 ,  C12P13/04

Abstract: An object of the present invention is to provide a method for producing an immobilized microorganism having high filtration properties, and to provide a method for producing an amino acid using the immobilized microorganism. A method for producing an immobilized microorganism is characterized in that a microorganism is contacted with carboxymethyl cellulose sodium salt and then contacted with polyethylenimine and an alkane dial after the first contact with carboxymethyl cellulose sodium salt.

公开(公告)号：US20170211116A1

公开(公告)日：2017-07-27

申请号：US15315116

申请日：2015-06-09

Applicant: Kaneka Corporation

Inventor： Hiroyuki KANAMARU ,  Teruaki TAKESUE ,  Osamu ODAWARA ,  Takeyuki TSUCHIDATE

IPC: C12P21/02

CPC classification number: C12P21/02 ,  C12N15/00 ,  C12N15/09

Abstract: The present invention provides a method for producing a recombinant protein, which can increase the cell growth rate in culture while stably maintaining plasmids in the cells, thereby improving the recombinant protein yield. The present invention provides a method for producing a recombinant protein, including a high-temperature culture step of culturing at 32° C. or higher a Brevibacillus bacterium that contains a gene encoding a recombinant protein, and a low-temperature culture step of culturing the Brevibacillus bacterium at a temperature lower than 32° C. after the high-temperature culture step.